(3)采用通用引物对获取的每个扁穗冰草幼嫩叶片cpdna的trnt-trnl片段进行pcr扩增,获得多个pcr扩增产物;将所有扩增产物经电泳检测纯化后再测序,获得每株扁穗冰草幼嫩叶片cpdna的trnt-trnl扩增片段的核苷酸序列数据;其中,扩增采用的通用引物为: trnt:5’-cattacaaatgcgatgctct-3’; trnl:5’-tctaccgatttcgccatatc-3’...
基于核糖体DNA的ITS序列和叶绿体trnT-trnL及trnL-trnF基因间区的菊花起源与中国菊属植物分子系统学研究[J].分子植物育种 2003.doi:10.3969/j.issn.1672-416X.2003.05.002赵惠恩,汪小全,陈俊愉,洪德元. 基于核糖体DNA的ITS序列和叶绿体trnT-trnL及trnL-trnF基因间区的菊花起源与中国菊属植物分子系统学研究(英文)[...
摘要: 目的 对245份不同产地羌活Notopterygium incisum样品的遗传多样性和遗传结构进行研究.方法 采用叶绿体cpDNAtrnT-trnL直接测序的方法和邻接法(NJ)对245份羌活样品进行遗传多样性和遗传结构分析.结果 物种水平上单倍型多样性(Hd)指数为0.873,核苷酸多样性(Pi)指数为0.004 ... 查看全部>> ...
目的 对245份不同产地羌活Notopterygium incisum样品的遗传多样性和遗传结构进行研究.方法 采用叶绿体cpDNAtrnT-trnL直接测序的方法和邻接法(NJ)对245份羌活样品进行遗传多样性和遗传结构分析.结果 物种水平上单倍型多样性(Hd)指数为0.873,核苷酸多样性(Pi)指数为0.004 07;种群水平上Hd介于0～0.900,Pi指数介于0～0.0544...
The cpDNA regions studied were intergenic spacers between trnL-trnF, trnT-trnL, trnH-psbA and trnL intron. We were able to identify a number of variable sites for cpDNA sequences of trnL intron, trnL-trnF IGS, trnT-trnL IGS, trnH-psbA IGS and intergenic transcribed spacer (ITS) regions ...
本发明方法包括:在扁穗冰草居群采集多株扁穗冰草幼嫩叶片;提取每个扁穗冰草幼嫩叶片总DNA;对每个扁穗冰草幼嫩叶片cpDNA的trnTtrnL片段进行扩增,获得多个扩增产物;经测序获得每株扁穗冰草幼嫩叶片cpDNA的trnTtrnL扩增片段的核苷酸序列;采用软件对序列数据进行分析;根据数据分析,获得扁穗冰草在不同地理种群cpDNAtrnTtrnL多态...
intergenic region of chloroplast (cp) trnT-trnLWang, HurngYiHsieh, ChiaHungHuang, ChinGiKong, SiuWahChang, HsiaoChiLee, HoHueiWang, WeiKuangChen, ShihLunTzeng, HsyYuWu, WenJer
cpDNA (petN-psbM/trnS-trnG/trnT-trnL) haplotype network and its spatial distribution in the Azores archipelago.Beatriz, Rumeu
The chloroplast DNA regions trnT-trnL was used to analyze to phylogenetic relationships and maternal donor of Kengyilia species and their closely related species. The Neighbor-Joining phylogenetic reconstructions partitioned the species into two reciprocally monophyletic groups. Kengyilia melanthera was ...
Fabaceae_TrnT-trnL_Edwards-etal-2008Data from: Plant DNA barcodes and the influence of gene flow.Naciri, YamamaCaetano, SofiaSalamin, NicolasNaciri, Y., Caetano, S., and Salamin, N. (2012). Plant DNA barcodes and the influence of gene flow. Mol. Ecol. Resour. 12, 575-580. doi: ...