Transfer RNAs (tRNAs) are a type of non-coding ribonucleic acid (ncRNA) that carry specific amino acids and recognize unique messenger RNA (mRNA) sequences. The pairing of a tRNA to its compatible mRNA sequence
Four triplets GUU, GUC, GUA, GUG code for the amino-acid valine. Two valine specific tRNAs have been separated by fractionation of mixedtRNA; tRNAis specific for GUand tRNAcorresponds to GU(see also ref. 1 for binding properties). Recent studies showed that although both species are ...
A specific cognate amino acid is charged or aminoacylated to each tRNA catalyzed by mt-aaRSs (17). We measured the mRNA expression levels of 14 genes encoding mt-aaRSs among the five tissues using quantitative PCR for these genes and 18S rRNA as normalization. The average mRNA level of eac...
These observations indicate that RNA cleavage is usually associated with a specific UUAA/U/G motif. Table 1. Sequences associated with major processing sites. RNA sequences immediately adjacent to the predicted processing sites are shown for each gene. Processing sites are written in bold type ...
Conclusions: Gene families evolving under concerted evolution are not amenable to classical phylogenetic analyses since paralogs maintain identical, species-specific sequences, precluding the estimation of correct gene trees from sequence differences. This leaves conservation of syntenic arrangements with ...
augmented by synthetic and viral RNA27. In a model system established for such analysis28(human TLR-transformed HEK293 cells), sup-tRNA did not activate TLR7 and only marginally activated TLR8 to the extent of the mock transfection (Fig.1e), suggesting that it might be a non-specific ...
Sulfur-containing nucleosides are post-transcriptionally introduced into tRNA molecules, where they ensure precise codon recognition or stabilization of tRNA structure, thereby maintaining cellular proteome integrity. Modulating sulfur modification controls the translation efficiency of specific groups of genes, ...
Transfer RNAs (tRNAs) are ubiquitous in all living organism. They implement the genetic code so that most genomes contain distinct tRNAs for almost all 61 codons. They behave similar to mobile elements and proliferate in genomes spawning both local and n
E. coli et al., E. coli Gln (Rossman fold) Gln Gln gaping cavern
(+) or without (−) CMC was reverse-transcribed with a primer specific to human mt-tRNACys. The sequence ladders for U and A were generated under the same conditions in the presence of ddATP and ddTTP, respectively. The positions for Ψs and sequence are shown with the gel image. ...