本研究首次在热带假丝酵母中开发了tRNA-gRNA策略,并基于该策略构建了CRISPR-dCas9基因调控系统.论文主要结果如下:(1)内源tRNA编码基因的挖掘.利用生物信息学工具tRNAscan-SE对热带假丝酵母ATCC 20336基因组序列进行分析,获得全基因组序列中tRNA的编码序列.选择一条长度为71 bp内源tRNAGly序列,与已报道的异源tRNAGly...
目的:探究巨细胞病毒(CMV)启动子驱动转运RNA-导向RNA(tRNA-gRNA)的表达是否能够在青鳉细胞系及胚胎中产生功能性的gRNA并诱导基因的突变。 创新点:利用CMV启动子驱动tRNA-gRNA的表达在青鳉细胞系及胚胎中产生功能性的gRNA,结合Cas9系统诱导了青鳉胚胎和细胞系中的基因突变,为在鱼类胚胎和细胞系中进行基因编辑提供了一...
one gRNA scaffold and one tRNAGlyare required). Unlike verified plasmids, the Golden Gate reaction mix also has a large number of unassembled fragments, and transforming these fragments may cause yeast
we developed the ABE8e with the polycistronic tRNA-gRNA expression cassette (PTG-ABE8e) and PTG-ABE7.10 and compared their A-to-G editing efficiencies using both transient and stable transformation in the allotetraploidNicotiana benthamiana. We found that the editing efficiency of PTG-ABE8e was si...
百度试题 题目RNA根据其功能和结构可以分为( ) A.mRNAB.tRNAC.dRNAD.rRNAE.gRNA相关知识点: 试题来源: 解析 ABD 反馈 收藏
百度试题 题目蛋白质合成过程中参与的RNA包括( )。 A.mRNAB.gRNAC.tRNAD.rRNA相关知识点: 试题来源: 解析 A,C,D 反馈 收藏
由于参与翻译DNA片段的tRNA的基本识别单位是3个碱基,因此如果被剪目标处,在修复过程中出现以3个碱基倍数的增减,则意味着原DNA翻译成蛋白质的过程仍将继续,只不过是相比原来增减对应数量的氨基酸。下列表述中,符合文意的一项是: A.DNA在修复过程中出现以3个碱基倍数的增减,会导致“翻译过程”被迫中断 B.通过gRNA与...
Poly-tRNA-gRNAEmbryosFish cellsGeneration of mutants with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is commonly carried out in fish species by co-injecting a mixture of Cas9 messenger RNA (mRNA) or protein and transcribed guide RNA (gRNA)...
本发明属于分子生物学领域,利用水稻的tRNA序列构建了tRNAgRNA系统,该系统在青鳉胚胎和细胞中能在RNA聚合酶启动子CMV的作用下产生具有功能性的gRNA,而不需要在体外先转录gRNA,从而与CRISPR/Cas9系统共同作用对青鳉基因组进行基因编辑.该方法证明了CMVtRNAgRNA序列在鱼类细胞和胚胎内能产生有作用的gRNA并和Cas9一起进行基因...
To ensure that all proteins and RNAs are expressed in one transcript, multiple gRNAs were constructed in a tandemly arrayed tRNA (transfer RNA)-gRNA architecture, and the triplex sequence was cloned between the protein-coding sequences and the tRNA-gRNA array. By leveraging this s...