The absence of tRNA thiolation leads to diminished translation elongation at AAA/CAA/GAA but not their synonymous codons, resulting in reduced levels of key proteins enriched in these codons, which are critical for appressorium development and function. Importantly, overexpressing these proteins can ...
当这一修饰过程---甲硫醇化（methylthiolation）---没有正确发生时，错误可能被纳入所产生的蛋白质中，这在人类中可能导致神经疾病、癌症和增加患2型糖尿病的风险。 论文共同通讯作者、宾夕法尼亚州立大学生化学家Squire Booker说，“甲硫醇化在细菌、植物和动物中普遍存在。在这项研究中，我们确定了一种叫做MiaB...
转移RNA(tRNA)是“读取”遗传密码的RNA,并将遗传密码翻译成氨基酸序列以制造蛋白质。在一些tRNA的特定位置添加一种化学标签---甲基硫基团,可以提高它们将信使RNA(mRNA)翻译成蛋白质的能力。当这一修饰过程---甲硫醇化(methylthiolation)---没有正确发生时,错误可能被纳...
转移RNA(tRNA)是“读取”遗传密码的RNA,并将遗传密码翻译成氨基酸序列以制造蛋白质。在一些tRNA的特定位置添加一种化学标签---甲基硫基团,可以提高它们将信使RNA(mRNA)翻译成蛋白质的能力。当这一修饰过程---甲硫醇化(methylthiolation)---没有正确发生时,错误可能被纳入所产生的蛋白质中,这在人类中可能导致神经疾...
来自宾夕法尼亚州立大学的Squire J. Booker课题组,解析了来自均匀拟杆菌的MiaB的几个不同状态结构,从而揭示了硫转移的机制,这一结果发表于近期的Nature,题为“Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB”。 作者首次解析了甲硫基转移酶与其大分子底物的复合物结构。
The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways. To study whether these are conserved among eukaryotes, we used gene shuffles to replace the yeast proteins by their human counterparts, hURM1 an...
在一些tRNA的特定位置添加一种化学标签---甲基硫基团,可以提高它们将信使RNA(mRNA)翻译成蛋白质的能力。当这一修饰过程---甲硫醇化(methylthiolation)---没有正确发生时,错误可能被纳入所产生的蛋白质中,这在人类中可能导致神经疾病、癌症和增加患2型糖尿病的风险。
produced in multimilligram quantities using expression vectors which overproduce the molecules in auxotrophic bacterial strains. Undermodified tRNAs will be prepared in a similar manner in auxotrophic strains which also lack activity for pseudouridilate synthase I (hisT), thiolation factor A for s4U (...
Of note, the (Fe–S)int is distinct from the sulfur intermediate called Sint, which is also made and exported by mitochondria but is instead used for cytoplasmic tRNA thiolation. In summary, our findings establish a direct and vital role of mitochondria in cytoplasmic iron–sulfur cluster ...
Two tRNAIle1 species from an extreme thermophile, Thermus thermophilus HB8: effect of 2-thiolation of ribothymidine on the thermostability of tRNA Biochemistry, 24 (1985), pp. 5711-5715 CrossrefView in ScopusGoogle Scholar 34 N. Shigi, T. Suzuki, M. Tamakoshi, T. Oshima, K. Watanabe Cons...