Nature Methods 12, 835–837 (2015) doi:10.1038/nmeth.3478 ARM-seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments Nature Methods 12, 879–884 (2015) doi:10.1038/nmeth.3508
推荐一篇Nature Chemical Biology的文章,“Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications”,通讯作者是Brigham and Women’s Hospital的Matthew K. Waldor和Satoshi Kimura 。 tRNA上的化学修饰...
大家好,今天分享一篇发表在JACS上的文章:Mass Spectrometry-Based Direct Sequencing of tRNAs De Novo and Quantitative Mapping of Multiple RNA Modifications,通讯作者是纽约理工学院的Shenglong Zhang教授,他们课题组主要研究RNA修饰的测序及其调...
Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered ...
Transfer RNAs (tRNA) are quintessential in deciphering the genetic code; disseminating nucleic acid triplets into correct amino acid identity. While this decoding function is clear, an emerging theme is that tRNA abundance and functionality can powerfull
研究者进一步应用RT-qPCR的方法检测m7G修饰对tRNA丰度的影响,结果发现包含m7G修饰的20种tRNA中有15种tRNA的丰度在敲除METTL1后显著下降,但回复METTL1后tRNA的丰度也随之回复,然而非m7G修饰的tRNA的丰度则不受影响。图1 METTL1介导的m7G修饰...
进一步对Dicer-like 1突变体的分析发现微小tRFs在突变体中的丰度下降了，暗示其生成可能与Dicer核酸酶相关（图2）。最后，研究人员开发了分析植物tRNA和tRFs的生物信息学工具tRFanalyzer，创建了模式植物拟南芥和水稻的tRFs数据库（http://www.biosequencing.cn/tRFanalyzer/），以便植物领域的科研人员对植物tRNA和tRFs的...
by lengthening the tRNA molecules with ligated adapter extensions, tRNAs could be sequenced, basecalled and mapped using biological nanopores92. However, in these studies, the proposed approach led to low sequencing yields of tRNA molecules (~20–40× lower than expected for a DRS run) and did...
K. Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications. Nat. Chem. Biol. 16, 964–972 (2020). CAS PubMed Google Scholar Suzuki, T., Nagao, A. & Suzuki, T. Human mitochondrial tRNAs: biogenesis, function, structural aspects, and diseases. Annu. Rev. ...
验证和重复实验(450k芯片和独立的靶向甲基化测序(targeted Bisuphite-sequencing))支持该结果。尽管在tRNA-iMet-CAT-1-4和tRNA-Ser-AGA-2-6中发生强烈的一致信号,但组织特异性是一个重要的驱动因素,与主要的细胞类型变化无关。本研究对人类基因组tRNA基因DNA甲基化状态进行了全面分析,揭示了随年龄增长而谨慎高的...