携带同种氨基酸而反密码子不同的tRNAs被称为“isoacceptors”;携带同种氨基酸且反密码子相同,但序列不同的tRNAs则互为“isodecoders”。最新的科研进展意外地发现tRNA isoacceptors和isodecoders具有特异性的、非被动参与翻译的主动调控功能,可以显著影响生物学过程和疾病进展。此外,tRNAs在体液中高丰度存在的特点使其...
携带同种氨基酸而反密码子不同的tRNAs被称为“isoacceptors”;携带同种氨基酸且反密码子相同,但序列不同的tRNAs则互为“isodecoders”。在真核生物中,isodecoders的数量变化很大,并且在进化过程中增加:例如,在人类中,有50%的tRNA基因编码isodecoders,而在...
携带同种氨基酸而反密码子不同的tRNAs被称为“isoacceptors”;携带同种氨基酸且反密码子相同,但序列不同的tRNAs则互为“isodecoders”。在真核生物中,isodecoders的数量变化很大,并且在进化过程中增加:例如,在人类中,有50%的tRNA基因编码isodecoders,而在...
Isolation of tRNA isoacceptors by affinity chromatography on immobilized bacterial elongation factor Tu. Anal Biochem. 1984 Jan; 136 (1):161–167.Derwenskus KH, Fischer W, Sprinzl M. (1984). Isolation of tRNA isoacceptors by affinity chromatography on immobilized bacterial elongation factor Tu. ...
A group of all tRNA isoacceptors carrying the same amino acid. tRNA isoacceptors Different tRNA species, which are aminoacylated with the same amino acid, but differ in their anticodon sequences. tRNA isodecoders tRNA species that are aminoacylated with the same amino acids and bear the same...
tRNA isoacceptors是指包括反密码子在内序列不同,但是能够转运相同氨基酸的tRNA;tRNA isodecoders是指具有相同的反密码子但其他序列不同的tRNA。干巴巴的文字看起来太过无趣且费解,还是给大家呈现图片以便于了解,这里还是以Ala-tRNA为例,具体如下图所示。 前面我们提到,Ala有3种tRNA,如上图Isoacceptor families一行...
tRNA isoacceptors是指包括反密码子在内序列不同,但是能够转运相同氨基酸的tRNA;tRNA isodecoders是指具有相同的反密码子但其他序列不同的tRNA。干巴巴的文字看起来太过无趣且费解,还是给大家呈现图片以便于了解,这里还是以Ala-tRNA为例,具体如下图所示。 前面我们提到,Ala有3种tRNA,如上图Isoacceptor families一行...
G Graphical summary of tRNA isoacceptors methylated by METTL1 (red) and non-methylated or non-transcribed (grey). Statistical tests: two-tailed Student’s t-test (C). ****p < 0.0001 Full size image Next, to precisely map m7G in tRNAs, we knocked out METTL1 in the PCa cell line...
• The most reliable tool for analyzing the tRNA anti-codon isoacceptors and isodecoders, applied withrtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit(Cat# AS-FS-004), a highly efficient system that removes methylations on the tRNA to obtain a new level of accuracy on the ...
A family of all tRNA isoacceptors carrying the same amino acid. Endonuclease An enzyme that hydrolyses the phosphodiester bond between two nucleotides in a sequence. RNA interference A process by which short RNA sequences block gene expression by binding to specific mRNAs to cause their destruction...