将cDNA克隆与中期染色体进行荧光原位杂交(FISH),可以在一个步骤中精确地进行染色体定位。此技术的综述,参见Verma等,Human Chromosomes:a Manualof Basic Techniques,Pergamon Press,New York(1988)。 一旦序列被定位到准确的染色体位置,此序列在染色体上的物理位置就可以与基因图数据相关联。这些数据可见于例如,V.Mckusick...
可用于染色体定位的其它类似策略包括原位杂交、用标记的流式分选的染色体预筛选和杂交预选,从而构建染色体特异的cDNA库。 将cDNA克隆与中期染色体进行荧光原位杂交(FISH),可以在一个步骤中精确地进行染色体定位。此技术的综述,参见Verma等,Human Chromosomesa Manualof Basic Techniques,Pergamon Press,New York(1988)。 一...
5)至有32P-Probe洗出前开始收集第一峰(可用Monitor监测)。 6)5滴/管,收集10-15管。 7)用液体闪烁仪监测同位素量 8)合并第一峰的收集液后即为所需制备的32P-Probe(第二峰为游离γ-32P-dATP)。 预杂交将样膜置于塑料袋中,加入3-10mg预杂交液(10×Denhardt’s;6×SSC,0.1mg/mlCT DNA(小牛胸腺DNA)。)...
Thus, to determine the subcellular distribution of ER stress-induced 5′-tRHs of tRNAGly(GCC), we conducted a fluorescent in situ hybridisation assay (FISH), with a probe designed to recognise 5′-tRHs of tRNAGly(GCC). This assay was performed under conditions designed to avoid the ...
Tissue microarray (TMA) and fluorescence in situ hybridization (FISH) TMA was produced from paraffin-embedded samples by Outdo Biotech (HLugA180Su04, Shanghai, China). The probe and the FISH Kit were ordered from Boster Biological Technology (Wuhan, China). Briefly, the TMA was dewaxed in ...
030. 25. Goodarzi H, Liu X, Nguyen HC, Zhang S, Fish L, Tavazoie SF. Endogenous tRNA- derived fragments suppress breast cancer progression via YBX1 displacement. Cell. 2015;161(4):790–802. https://doi.org/10.1016/j.cell.2015.02.053. 26. Pekarsky Y, Balatti V, Palamarchuk A, ...
Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eu
Labelling of sRNA with fluorescent dye tsRNA and siRNA against EGFR were labelled with fluo- rescent dye by FISH Tag RNA Multicolor Kit (Invitrogen) according to manufacturer's instructions. Cells were fixed by paraformaldehyde (3%) 24 h post-transfection followed by confocal...
In another embodiment, nucleic acid probes derived from the instant nucleic acid sequences may be used in direct fluorescence in situ hybridization (FISH) mapping (Trask (1991)Trends Genet.7:149-154). Although current methods of FISH mapping favor use of large clones (several to several hundred...
030. 25. Goodarzi H, Liu X, Nguyen HC, Zhang S, Fish L, Tavazoie SF. Endogenous tRNA- derived fragments suppress breast cancer progression via YBX1 displacement. Cell. 2015;161(4):790–802. https://doi.org/10.1016/j.cell.2015.02.053. 26. Pekarsky Y, Balatti V, Palamarchuk A, ...