The stRNA shares significant sequence homology with natural tRNA, but its anticodon can specifically identify termination codons (UAG, UGA, UAA) via base pairing. When the ribosome encounters an early termination codon during translation, stRNA inserts the corresponding amino acid into the peptide ...
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especially those found at position 34 of the anticodon16,32,33,34, restricting or increasing the wobbling capacity of the tRNAs and, consequently, changing the set of ‘preferred’ or ‘optimal’ codons that will be translated35,36,37,38,39,40,41,42,43,44. In the last few years, it h...
The role of transfer RNAs (tRNAs) in deciphering the genetic code is universal to cell biology. The trinucleotide anticodon sequence of each tRNA-type decodes specific codons employed by messenger RNAs (mRNAs). Overall, the number of genes encoding each tRNA-type and the relative cellular abunda...
Transfer RNAs (tRNAs) are among the most abundant non-coding RNAs (ncRNAs), constituting 4–10% of all cellular RNAs [1]. tRNAs play crucial roles in protein translation by recognizing mRNA codons and transferring specific amino acids to ribosomes for polypeptide chain elongation. Beyond their ...
and Ernst, Joachim F., "Toxicity of a Heterologous Leucyl-tRNA (anticodon CAG) in the Pathogen Candida albicans: In Vivo Evidence for Non-Standard Decoding of CUG Codons," Mol. Gen. Genet., 245:212-217 (1994). Houman, F., et al., "Cloning, Expression and Characterization of Iso...
said source cell, and (h) engineering a polynucleotide sequence for the expression of said protein in said destination cell, wherein said engineering excludes codons that correlate to anticodons of tRNAs in the destination cell that are present in an amount that is less than in said source ...
These observations indicate that it should be possible to create a cell that contains both of these two different orthogonal HisRS/tRNAHis pairs. As the anticodon of the tRNAHis with GÀ1 is not recognized by the GÀ1 type HisRS, its backbone can be utilized to constru...
Transfer RNAs (tRNAs) play an essential role in mRNA translation by delivering amino acids to growing polypeptide chains. Recent data demonstrate that tRNAs can be cleaved by ribonucleases, and the resultant cleavage products, tRNA-derived small RNAs (ts