Like N6amt1, all the three bacterial DNA 6mA MTases belong to the class I MTase family28. The MTase domain of these enzymes can be superimposed well with that of N6amt1 (an RMSD of 3.0 Å for 140 Cα with Dam; an RMSD of 3.3 Å for 50 Cα with RsrI; an RMSD ...
DNA adenine-N6 and cytosine-N4 MTases, and protein or small molecule MTases2. In essence, the two most conserved sequence motifs reflect the common functions of the MTases: motif I (FxGxG) for binding of the methyl donor SAM and motif IV for ...
Interestingly, bioinformatic analyses of the sequence of the MTase domains fused to Trm112 reveal that these MTases do not all belong to the same family and that some of these are putative archaeal orthologs of Trm9. Finally, no protein with significant sequence similarity with Trm112 could be...