TROUBLESHOOTING Problem:RNA yield is low. [Step 13] Solution:Unexpectedly low RNA yield can occur when the initial solubilization step is not effective; e.g., it is too short or not enough TRIzol has been used. Make sure that the solution is clearand of uniform viscosity. INTRODUCTION TRIzol...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. Most likely the sample was homogenized in an insufficient volume of TRIzol Reagent. Make sure to add the appropriate amount of TRIzol Reagent for your sample type. ...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. Add 10 micrograms of RNase-free glycogen to less than 10 mg tissue or less than 1 X 10e6 suspension cells. Glycogen, unlike salmon sperm DNA carrier, can be added...
没事的，TRizol的主要成分是异硫氰酸胍和苯酚，对于皮肤的损伤主要是苯酚对于皮肤的腐蚀，不懂的话，参见...
以前提取RNA的时候，尽管带着手套操作，但还是不可避免的会有TRizol溅到皮肤上，一般洗一下手，就没事...
Troubleshooting Observation A lower yield than expected is observed The sample is degraded The RNA or DNA is contaminated The RNA A260/280 ratio is low The DNA A260/280 ratio is low Possible cause The samples were incompletely homogenized or lysed. The pellet was incompletely solubilized Samples...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. Blood collected with EDTA typically has the highest DNA contamination, blood collected with heparin typically has less than that collected with EDTA, and blood collected ...
这个没事的吧，不用过分担心的
trizol就是主要苯酚，屁事没有，就是刺激腐蚀皮肤罢了 ~~桐桐爱你爱你 ️ ️ ️ ️...
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center Blood collected with EDTA typically has the highest DNA contamination, blood collected with heparin typically has less than that collected with EDTA, and blood collected with citrate...