Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. There are several possible stopping points and recommended storage conditions during the extraction of RNA with TRIzol Reagent: ...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. This is most likely polysaccharides or cell membranes; DNA should be in the interphase. In samples containing blood (e.g., liver), a red viscous layer may be visib...
TroubleshootingProtein resuspension, continuedPoor solubility of the pellet in SDS can occur, because the solubility of specific classes of proteins differs with different solvents. If the protein pellet is insoluble in SDS, the following alternative solvents (Hummonet. al., 2007) maybe required to ...
TROUBLESHOOTING Problem:RNA yield is low. [Step 13] Solution:Unexpectedly low RNA yield can occur when the initial solubilization step is not effective; e.g., it is too short or not enough TRIzol has been used. Make sure that the solution is clearand of uniform viscosity. INTRODUCTION TRIzol...
Troubleshooting Observation A lower yield than expected is observed The sample is degraded The DNA is contaminated The DNA A260/280 ratio is low Table 3 Typical DNA (A260/280 of 1.6–1.8) yields from human leukocytes Starting material Human leukocytes Quantity 7 × 108 cells DNA yield 1.3 mg...
or store the sample at –20°C.TroubleshootingProtein resuspension, continuedPoor solubility of the ...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. There are several possible stopping points and recommended storage conditions during the extraction of RNA with TRIzol Reagent: ...
Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. There are several possible stopping points and recommended storage conditions during the extraction of RNA with TRIzol Reagent: ...
or store the sample at –20°C.TroubleshootingProtein resuspension, continuedPoor solubility of the ...
Degraded RNA can cause an increased absorbance at 260 nm. Find additional tips, troubleshooting help, and resources within ourRNA Sample Collection, Protection, and Isolation Support Center. This is most likely polysaccharides or cell membranes; DNA should be in the interphase. In samples containing...