TRIzol Reagent RNA的提取 准备试剂：氯仿，异丙醇，75℅乙醇，无RNase的水或0.5℅SDS(溶液均需用DEPC处理过的水配制) 操作步骤: 1.匀浆处理:a.组织将组织在液氮中磨碎,每50-100mg组织加入1mlTRIzol,用匀浆仪进行匀浆处理。样品体积不应超过TRIzol体积10℅。 b.单层培养细胞直接在培养板中加入TRIzol裂解细胞，...
attention if necessary. If you inhale vapors, move to fresh air and seek medical attention if necessary. For more information, refer to the TRIzol® Reagent SDS (Safety Data Sheet), available from our website atwww.invitrogen.com/sds. Contents and Storage TRIzol® Reagent is supplied in 1...
SDS Product Information User Guide: TRIzol LS Reagent Manual: TRIzol LS Reagent (Japanese) User Guide: TRIzol LS Reagent - Experimental protocol for DNA isolation 常見問答集 (常見問題) Blood collected with EDTA typically has the highest DNA contamination, blood collected with heparin typically has ...
1)取一定量的纯化过的孢子悬浮液,12000rpm ,离心5min,弃上清 a 或者用匀浆仪进行匀浆处理,悬浮液不经离心,直接加0.5ml Trizol reagent于冰上充分匀浆悬浮液样品体积一般不要超过Trizol体积的10%.,然后再加入0.5ml Trizol 冲洗匀浆器,转移至1.5ml EP管中 b 直接在悬浮液中加入Trizol裂解细胞,加1ml Trizol.用取...
4 TRIzol™ LS Reagent User Guide 4. Transfer the supernatant containing the proteins to a new tube. 5. (Optional) Solubilize the pellet by adding 100 µL of 1% SDS and 100 µL of 8 M urea. Proceed directly to downstream applications, or store the sample at –20°C. ...
TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
contact to skin or eyes occurs, immediately wash the exposed area with copious amounts of water for 15 minutes and seek medical attention if necessary. If you inhale vapors, move to fresh air and seek medical attention if necessary. For more information, refer to the TRIzol® Reagent SDS ...
简要描述:Invitrogen原装TRIZOL REAGENT(货号:15596-026)100ML 慧颖*中TRIzol® 试剂是一种*的即用型试剂,可用于提取高质量的总RNA或者从各种生物样本中同时提取RNA、DNA和蛋白质。这种酚和异硫氰酸胍单相溶液,可用于提取人、动物、植物、酵母或细菌来源的细胞和组织样本中的纯RNA、DNA和蛋白质,只需一个小时即可...
英文名称:TRIzol® Reagent 保质期:2年 个月 保存条件:2-8度 名称:TRIzol® Reagent 15596026 现货 货号:15596026 规格:100 mL 产品优点:可快速简单地获得高纯度总RNA 样品类型:细菌,血液,植物样品,组织,细胞,酵母,病毒样本。 保存温度:2-8度
含0.5% SDS的RNase-free水或RNase-free水或DEPC处理水 【可选】RNase-free的糖原(核酸助沉剂) 2. 样本要求 【重要】:样本收集后立即进行RNA分离,或样本收集后立即冻存样本并保存在-80℃或液氮中直至RNA抽提。 样本类型 每mlTRIzol Reagent处理的起始样本量 组织(新鲜组织或保存在RNAlater等稳定液内的组织...