TRIzol™ LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol™ LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an int...
TRIzol™ Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase...
TRIzol®reagent is an acid-guanidinium-phenol based reagent ideally designed for the extraction of RNA (as well as DNA and protein) from various biological sample inputs. The low pH (acidic) of TRIzol®controls to separate RNA from DNA and protein, while a high pH can cause RNA and DNA...
(Chomczynski, 1993). After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated fro...
TRIzol® Reagent allows the user to perform sequential precipitation of RNA, DNA, and proteins from a single sample (Chomczynski, 1993). After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (...
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After centrifugation the colorless upper aqueous phase is transferred to a fresh tube, and RNA is precipitated by mixing it with 2-propanol (0.5 ml/ml of TRIzol reagent used initially), incubated at 25° for 10 min, and centrifuged at 12,000 g for 10 min at 5°. The gel-like pellet ...
TRIzol™ Reagent allows to perform sequential precipitation of RNA, DNA, and proteins from a single sample (Chomczynski, 1993). After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA)...
Reagent®.6These reagents lyse cells, release RNA, and prevent RNA degradation by inhibiting (most) RNases. They also separate lysate into different phases, enabling two other protocols (hence the “tri-“ prefix) using the interphase and organic phase for genomic DNA and protein purification,...
The organic phase can be stored at 4°C overnight. Lyse samples and separate phases 1. Add 0.75 mL of TRIzol™ LS Reagent per 0.25 mL of sample volume. 2. Homogenize the sample by pipetting up and down several times. Note: The sample volume should not exceed 10% of the volume of ...