Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling–heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of ...
In this paper, the cloud point extraction and separation of scandium and yttrium (III) via use of Triton X‐114 with and without 8‐hydroquiloline (HQ) as an added chelate agent are investigated. The effects of various parameters, such as the aqueous phase pH, HQ concentration, Triton X‐...
Triton X-114 phase partitioning of a nonlipidated form of the 17-kDa immunogen cleaved from a glutathione S-transferase fusion protein demonstrated that ... D R,Akins,B K,... - 《Infection & Immunity》 被引量: 146发表: 1993年 Characterization of a soluble, catalytically active form of Esc...
Analysis of sulfuric acid biosynthesis enzyme genes Many marine gastropod snails, for the purpose of feeding and defense, release strong acids20,21,113,114,115,116. Although acids can facilitate penetration through the prey calcareous body wall, the acids can also serve as an allomone through the...