1、Tris缓冲盐溶液(TBS)(Tris-Buffered Saline)载体酶切:将表达质粒用限制性内切酶(同引物的酶切位点)进行双酶切,酶切产物行琼脂糖电泳后,用胶回收Kit或冻融法回收载体大片段。 2、PCR产物双酶切后回收,在T4DNA连接酶作用下连接入载体。 (三)获得含重组表达质粒的表达菌种 ...
Tris buffered saline (TBS) buffer is commonly used as a wash and/or dilution solution in a variety of applications including cell culture, Western blotting, ELISA, and immunohistochemistry. Takara Bio offers both tablets and powder for easy preparation of TBS buffer. Tablets are available for TBS...
Tris Buffered Saline(TBS)(Tris缓冲盐水),20X 英文名称:Tris Buffered Saline,20X 运输:常温 保存:4-25℃ 有效期:2年 货期:1-3天 本公司定制各种即用型溶液欢迎咨询,PH可定制。 与Tris Buffered Saline,20X相关的更多产品: Tricine-SDS Sample Buffer(Tricine-SDS PAGE胶样品缓冲液),2X,非还原型 Tricine-SDS...
Medicago's TBS buffer is supplied as pre-weighed tablets in bottles and in blister packs or as powder in sealed pouches. One tablet or the contents of one pouch gives 500 ml or 1000 ml of buffer solution in the compositions: 1. 1 x solution; 0.05 M Tris buffered saline, 0.138 ...
产品名称 :PH1947 | TBS缓冲液 Tris Buffered Saline 产品品牌 :飞净 PHYGENE TBS即Tris Buffered Saline。TBS (Tris Buffered Saline),中文名为Tris缓冲盐溶液、Tris盐缓冲液、TBS缓冲液,是生物学中常用的缓冲液,广泛用于Western、免疫组化、免疫沉淀、免疫共沉淀、ELISA等实验中洗涤缓冲液或封闭液等的配制。TBS由...
Tris Buffered Saline(TBS)(Tris缓冲盐水),10X KL-25-07410 250ml 390元 英文名称:Tris Buffered Saline 运输:常温 保存:4-25℃ 有效期:2年 货期:1-3天 其他: 产品介绍: 本公司定制各种即用型溶液欢迎来电咨询。 pH缓冲液显示pH跟正常值差距过大 ...
JAW™ Tris Buffered Saline (TBS) Packs are ready to use dry-blend powder pouches designed to make 1X solution of TBS. Just Add Water! Features: Each Pack is dissolved in 1L deionized water to make 50mM Tris, 138mM sodium chloride, 2.7mM potassium chloride, pH 7.90-8.20 Come in conven...
Tris Buffered Saline (TBS) is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alka...
Tris buffered saline (TBS) solution with the detergent Tween 20 for use as a wash buffer during western blotting. Product is shipped and stored at room temperature. 1X Formulation: 137 mM Sodium Chloride, 20 mM Tris, 0.1% Tween-20. Supplied at pH 7.6. 限制使用 除非CST 的合法授书代表以...
名称编号规格价格手册 Tris-Buffered Saline (TBS) (10x)6-3525250mL995 英文名称:Tris-Buffered Saline (TBS) (10x) 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍: Order Online在线订购 订货信息 产品名称* 申请类型请选择类型产品订购试用索取* ...