pH should be 7.4, if not adjust with 1.0N NaOH or 1.0N HCl (3 Liters-13.25g Mono and 67.52g Dibasic) 0.1M Phosphate Buffer Saline (PBS)-8 Liters: Prepare 4 liters of 0.2M phosphate buffer (see above recipe) Add 72g NaCl (0.9% or 9g/liter) ...
与 NuPAGE Bis-Tris 凝胶类似,Bolt Bis-Tris Plus 预制胶旨在在中性 pH 环境中提供一致的凝胶性能,从而尽量减少蛋白质修饰并提高上样容量。Bolt 凝胶是蛋白质免疫印迹转印和分析以及任何其他需要蛋白质完整性的技术的理想选择。 使用Bolt Bis-Tris Plus 预制胶可获得更佳结果,以满足日常蛋白分离需求。Bolt Bis-...
与传统 Tris- 甘氨酸预制胶不同,NuPAGE Bis-Tris 预制胶具有中性 pH 环境,可尽量减少蛋白质修饰。NuPAGE Bis-Tris 凝胶适用于制备蛋白用于测序、质谱以及其他任何蛋白完整性至关重要的技术。 NuPAGE Bis-Tris 预制胶的特点包括:•更出色的蛋白完整性—优化的样品前处理过程,完整保护您的蛋白质• 更宽的分子...
Buffer (20X) and NativePAGE™ Cathode Buffer Additive (20X) for native electrophoresis • NuPAGE® Transfer Buffer for blotting of NativePAGE™ Novex® Bis-Tris Gels NativePAGE™ Novex® Bis-Tris Gels The NativePAGE™ Novex® Bis-Tris Gel is a 1.0 mm thick, 8 x 8 cm mini...
Proceed with the transfer using regular Tris-Glycine transfer buffer. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center. Here are possible causes and solutions: - Buffers are too concentrated or incorrect. Check buffer recipe...
NuPAGE Tris 醋酸盐蛋白凝胶的聚丙烯酰胺浓度为 7%,梯度为 3–8%。凝胶有两种规格:小型凝胶 (8 cm x 8 cm) 或中型凝胶 (8.7 cm x 13.3 cm),厚度为 1.0 mm(小型和中型凝胶)或 1.5 mm(仅限小型凝胶)。NuPAGE Tris-乙酸预制胶还提供多种孔规格。小型凝胶可使用我们的 XCell SureLock 小型电泳槽 (EI...
1) Buffers are too dilute: Check buffer recipe; remake if necessary. 2) Upper buffer chamber is leaking: Make sure the buffer core is firmly seated, the gaskets are in place and the gel tension lever is locked. 3) Voltage is set too low: Set correct voltage. Find additional tips, tro...
与传统 Tris- 甘氨酸预制胶不同,NuPAGE Bis-Tris 预制胶具有中性 pH 环境,可尽量减少蛋白质修饰。NuPAGE Bis-Tris 凝胶适用于制备蛋白用于测序、质谱以及其他任何蛋白完整性至关重要的技术。 NuPAGE Bis-Tris 预制胶的特点包括:•更出色的蛋白完整性—优化的样品前处理过程,完整保护您的蛋白质• 更宽的分子...
Check buffer recipe; dilute or re-make if necessary. - Voltage, current or wattage is set at a higher limit. Decrease power conditions to recommended running conditions. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center. ...