We used three approaches to validate the identity of each PDX model. First, we confirmed the human origin of each PDX tumor by quantifying the ratio of human-to-mouse genomic DNA in PDX tumors as described previously12. Briefly, this was done using qPCR with a human RNaseP gene probe (20...
BRCA1 and Rad51C methylation, bisulfite sequencing The promoter region ofBRCA1andRAD51Cwas identified using the Eukaryotic Promoter Database (http://epd.vital-it.ch/index.php).BRCA1promoter was amplified with forward-TATTTTGAGAGGTTGCTGTTTAG and reverse-CTAAAAAACCCCACAACCTATCCC primers. Analysis...
Triple-negative breast cancer: Role of specific chemotherapy agents. Cancer J. 2010, 16, 53–61. [Google Scholar] [CrossRef] [PubMed] Liedtke, C.; Mazouni, C.; Hess, K.R.; André, F.; Tordai, A.; Mejia, J.A.; Symmans, W.F.; Gonzalez-Angulo, A.M.; Hennessy, B.; Green,...