TMP-NHBocis deprotected to yield reactiveTMP-NH2, which is then linked toLumi4-NHS(Fig. 17.2) (1day) ∘ In round-bottom flask, combine ∼ 2 mg (3 μmol, 1.5 equiv.)TMP-NHBocwith 2–3 mL TFA/CH2Cl2(50%, v/v) and stir for 5 h at room temperature. Evaporate solvent and ...