2 结果 2.1 TRIM32基因克隆 从草鱼脾脏组织中提取总RNA, 以反转录获取 的cDNA为模板, 扩增TRIM32 CDS区全长.经琼脂 糖凝胶电泳检测, 结果显示均在大约2000 bp位置检 测到特异性目的条带(图 1).将扩增片段纯化回收 连接到克隆载体pMD19-T得到重组质粒pMD19-T- TRIM32, 经测序草鱼TRIM32 CDS区全长为1980
TRIM25 CDS was amplified and then inserted into the pEGFP-C1 vector between HindIII and BamHI restriction sites, named pEGFP-C1-T25 (OverTRIM25). A specific small interfering RNA (siRNA) against TRIM25 (siTRIM25) and the corresponding negative control oligonucleotide (siNC) purchased from Gene...
To investigate the function ofMs2gene, we identified the differentially expressed (DE) genes between the fertile and sterile anthers using RNA-Seq transcriptome analysis. A total of 4,814 genes were found to be differentially expressed between fertile and sterile anthers (a minimum of one fold ...
Some TRIMs are known to have the function of both positive and negative regulation of the same cell signal transduction pathway. TRIM38 is a representative member of the group, which play an inhibitory role in the TLR3/TLR4 pathway and a synergistic effect on the RIG-I pathway [12]. ...
In addition, use of a phase shift mask: increases the usable process window at very small CDs, increases the range of CDs available from a single mask through overexposure or underexposure, and reduces the mask error function as compared to conventional binary masks. For example, at least one...
Note protruding rod, far right bottom corner, which performs the reset function when the back is closed. The actual frame counting is done by a toothed wheel on a spring loaded shaft in the film take up chamber. Once start marks appeared, they were present in all bodies, including in ...
The mechanism of RBS-mediated transcriptional repression is unknown but is thought to act as a DNA element in the context of the integrated provirus. Therefore, RBS function is thought to be distinct from the role of the PBS in tRNA annealing and priming on the viral RNA. Evidence has been...
Here, we describe the structure and function of a novel RBCC/TRIM-B30.2 protein, Bloodthirsty (Bty), and demonstrate that it is required for erythropoiesis in the zebrafish. The bloodthirsty cDNA/gene (bty) was discovered as one of several B30.2-encoding cDNAs that were related to a subtracti...