real-time quantitative PCR analysis and ELISA detection showed that the synthesis and secretion of insulin were hindered. We used Trim-Way technology to specifically eliminate the excessive accumulation
Similar content being viewed by others Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology ArticleOpen access23 January 2019 Stable maternal proteins underlie distinct transcriptome, translatome, and proteome reprogramming during mouse oocyte-to...
Trim-Away is a recently developed technology that exploits off-the-shelf antibodies and the RING E3 ligase and cytosolic antibody receptor TRIM21 to carry out rapid protein depletion. How TRIM21 is catalytically activated upon target engagement, either during its normal immune function or when repurp...
TRIM21胞内免疫功能与治疗应用
MOE Key Laboratory for Cellular Dynamics, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, P.R. China Qiong Guo, Xinyan Chen & Chao Xu Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney ...
The cytosolic antibody receptor TRIM21 possesses unique ubiquitination activity that drives broad-spectrum anti-pathogen targeting and underpins the protein depletion technology Trim-Away. This activity is dependent on formation of self-anchored, K63-linked ubiquitin chains by the heterodimeric E2 enzyme ...
[英 [trɪm] 美 [trɪm] ] trim的意思、解释 过去式:trimmed; 过去分词:trimmed; 现在分词:trimming; trim 基本解释 及物动词装饰; 修剪; 整理 形容词整齐的,整洁的; 修长的; 苗条的 名词修剪; 整齐; 健康状态; 装束 不及物动词削减 trim 相关例句 ...
He is also member of the European Association for Signal Processing (EURASIP), the German Information Technology Society (ITG) in the Association for Electrical, Electronic and Information Technologies (VDE) and the Austrian Electrotechnical Association (ÖVE)....
SPOP Y87C/F133V/W131G mutants were generated by QuickChange II site-directed mutagenesis kit (Aligent Technology) with MYC-SPOP WT as a template. All the plasmids were verified by sequencing. HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712). pKH3-TRIM28 was a gift ...
The trim strip is retained in wrapping engagement on the rotary surface by pressure differential and thereby positively transported away from the severance point into a chute. At a point spaced from the severance point the trim strip is stripped from the rotary surface for onward movement in the...