As for the GI50 determination, additional cell viability was obtained at the time point of compound treatment (t = 0). For the proliferation curve of Dox-induced HsClpP overexpression, the cells were seede
1). To determine whether CD8+ T cells contribute to oligodendrocyte and myelin pathology in 5xFAD mice, we performed antibody-mediated CD8+ T cell depletion for 6 weeks starting in 6-month-old 5xFAD mice, a time point when CD8+ T cells were starting to appear in the GM. The treatment ...
To obtain sufficient RNA for every time point, thymi of several mice were pooled. All samples underwent a quality control on a bioanalyzer to exclude degradation of RNA. GSEA analysis was performed using the GSEA tool v4.1 of the Broad Institute (https://www.gsea-msigdb.org/gsea). ...
At the indicated time point, femurs from mice with or without intestinal inflammation were homogenized and assessed for cytokine abundance via Luminex profiling. Values were normalized to total protein as measured by a Pierce bicinchoninic acid (BCA) protein assay. Cytokines that were significantly ...
Long noncoding RNAs (lncRNAs) have emerged as critical players in cancer progression, but their functions in colorectal cancer (CRC) metastasis have not been systematically clarified. lncRNA expression profiles in matched normal and CRC tissue were check
Worms were washed with M9 buffer at respective time points and sorted using COPAS biosorter to enrich for a synchronized population for each time point. CSR-1 expression was monitored using live imaging. Brood-size assay For the brood size, single L1 larvae were manually picked and placed onto...
Moreover, within the first few days after birth (while located inside the GCL), newborn cells start receiving synaptic GABAergic input, presumably from local interneurons in the GCL, but not glutamatergic inputs, consistent with the absence of dendritic spines in the ML at this time point28,...
Each system was placed in a cubic periodic box and solvated with the simple point charge (SPC) model using a distance of 0.14 nm between the molecule and the wall of the box (Berendsen et al., 1981). All molecular dynamics (MD) simulations were performed at constant temperature (298 K)...
point of the experiments. Mock-transfected cultures served to set the thresholds for background fluorescence (negative control).dGene targeting in cells containing donor DNA resistant and susceptible to RGN nicking. Amplicons diagnostic for homology-directed gene targeting involving EGFP-encoding ...
Mice were monitored by weight and were culled if they reached their humane end point: 70% of maximum weight (survival = time to humane end point or death, whichever comes first). For wide-field calcium imaging in neurons, we generated conditional α2-Na/K ATPase knockout mice with G...