The tri-snRNP component Prp31 interacts indirectly with Mtr4 and PM/Scl-100 in a manner that is dependent on the phosphorylation sites in the middle of the protein, while Prp3 and Prp4 interact with the nuclear decay complex independent of Prp31. Together our results suggest recruitment of ...
U4/U6.U5 tri-snRNP是剪接体组装过程中最大也是最保守的预组装复合物,它由U5小核核糖核蛋白(snRNP),U4、U6 snRNA和其他蛋白因子组成。在该复合物中,U6 snRNA呈现失活构象。U4/U6.U5 tri-snRNP与A复合物结合形成B复合物,再经过一系列成分、构象重组后,U1、U4 snRNP解离,剪接体形成,U6 snRNA从而被激活。所以...
该研究报道了一株酿酒酵母 U4 /U6.U5tri-snRNP 的三维结构,其总分辨率为 3.8 埃。Tri-snRNP 核心区的局部分辨率达到 3.0 到 3.5 埃,可以构建精细的原子模型。 作者解析的结构包括 U5snRNA、广泛碱基配对的 U4 /U6snRNA 以及包括 Prp8 和 Snu114 在内的 30 个蛋白质,共计 8495 个氨基酸和 263 个核苷酸,...
U4/U6.U5 Tri-SnRNP相关蛋白1(SART1)抗体 英文名称: U4/U6.U5 Tri-SnRNP-Associated Protein 1 (SART1) Antibody 品牌: Abbexa 货号: abx431570 规格: 200 µl 价格: $398.75 产品说明书下载: 产品预定 >> 产品预定,提交订单信息之后,我们会第一时间与您取得联系 一抗一...
U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing,
Here we identify two previously unidentified phosphoproteins in the human spliceosomal B complex, namely the pre-mRNA processing factors PRP6 and PRP31, both components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP). We provide evidence that PRP6 and PRP31 are directly phosphorylated...
Tri-snRNP docking involves the formation of U2/U6 helix II. This interaction is stabilized by a 5′ splice site (SS)-containing oligonucleotide, which can bind the tri-snRNP and convert the cross-exon complex into a cross-intron, B-like complex. Our data suggest that the switch from cross...
SART1 (Squamous Cell Carcinoma Antigen Recognized by T-cells 1, SART-1, hSART-1, Allergen=Hom s 1, Ara1, HOMS1, SART1259, SNU66 Homolog, hSnu66, U4/U6.U5 tri-snRNP-associated Protein 1, U4/U6.U5 Tri-snRNP-associated 110kD Protein, SNRNP110)化学性质...
The association of the U4/U6.U5 tri-snRNP with pre-spliceosomes is a poorly understood step in the spliceosome assembly pathway. We have identified two human tri-snRNP proteins (of 65 and 110 kDa) that play an essential role in this process. Characterization by cDNA cloning of the 65 and...
Interestingly, the U5-102K protein has been shown previously to play an essential role in tri-snRNP formation, binding the U4/U6-61K protein. The interaction of 52K with a tri-snRNP bridging protein, coupled with its absence from the tri-snRNP, suggests it might function in tri-snRNP ...