After purification via single spore isolation, the proper insertion of the replace- ment cassette was verified by Q 5®-PCR with primer pair At-HDR_Diag (Additional file 1: Table S1) which ampli- fie
After purification via single spore isolation, the proper insertion of the replacement cassette was verified by Q5®-PCR with primer pair At-HDR_Diag (Additional file 1: Table S1) which amplifies the region that spans from outside of the homology regions and across the whole insert. The ...