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and high-throughput manner. We demonstrate the application of CRISPR-AID not only to increase the production of β-carotene by 3-fold in a single step, but also to achieve 2.5-fold improvement in the display of an endoglucanase on the yeast surface by optimizing multiple metabolic engineering ...
comparable mM concentrations (Supplementary Fig.6). Importantly though, only the intracellular concentration of magnesium is universally held at mM concentrations, whereas the intracellular concentration of the metals are 103to 106-fold lower26, indicating that the other metals will not be inhibitory in...
On this basis, we continue to screen the white-backed planthopper under a low dose of triflumezopyrim, with 20 generations screened thus far; the resistance ratio (RR) has reached 20.9-fold. Our objective is to determine the relative expression level of the ultraspiracle (USP) gene from tr...
4.6. Cell-Free In Vitro Translation and Translocation The DNAJC3 SP and first 62AA of mature DNAJC3 were fused upstream of huCD4 with PCR. The Qiagen EasyXpress linear template kit was used to generate linear DNA fragments using PCR. The PCR products were purified and transcribed in vitro...