Background: Human telomeres are coated by the telomere repeat binding proteins TRF1 and TRF2, which are believed to function independently to regulate telomere length and protect chromosome ends, respectively. Results: Here, we show that TRF1 and TRF2 are linked via TIN2, a previously ...
Highlights ► Role of phosphorylation in regulating human telomere binding proteins TRF1 and TRF2. ► Role of ubiquitylation/de-ubiquitylation in regulating TRF1 and TRF2 stability. ► Role of other modifications in regulating TRF1 and TRF2 function.Access...
Looking for online definition of TRF1 in the Medical Dictionary? TRF1 explanation free. What is TRF1? Meaning of TRF1 medical term. What does TRF1 mean?
Increased expression of telomere length regulating factors TRF1, TRF2 and TIN2 in patients with adult T-cell leukemia Here, we report that freshly isolated unstimulated adult T-cell leukemia (ATL) cells present high telomerase activity compared to asymptomatic carriers or ... M Bellon,A Datta,M...
Wild-type TRF1 and TRF1 mutants (T371A and T371D) were then subcloned into either the retroviral vector pWZL-N-myc35 or the bacterial expression vector pHis-Parallel-2 (ref. 36). The annealed oligonucleotides encod- ing shTRF1 (5′-GAATATTTGGTGATCCAAA-3′) were ligated into pRetro...
Telomeric repeat binding factor 1 (TRF1) and 2 (TRF2) may play key roles in the maintenance of telomere function. TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions. We examined the expression of TRF1 and TRF2 in 20 ...
Residues with a selective function in both TRF1 TRFH and TRF2 TRFH and forming a stable hydrogen bond network with TIN2 TBM or Apollo TBM peptides were traced. Our study revealed that TIN2 TBM forms a well-defined binding mode with TRF1 TRFH as compared to TRF2 TRFH , and that the ...
Although no clear telomere shortening or elongation was observed in short term cultured TRF1-deficient cells, abnormal telomere signals were observed, and TRF1-interacting telomere-binding factor, TIN2, lost telomeric association. Furthermore, another double-stranded telomeric DNA-binding factor, TRF2,...
Thus, the homodimerization, as mediated by the TRFH domain, is likely to be a crucial aspect of the function of these proteins. Here, we present the 3D structure of the dimerization domain from both human TRF1 and TRF2. Despite a modest sequence conservation, the two domains have the same...
Manipulation of the telomere-binding protein gene expression reveals that alterations in those proteins can impair the function of the telomere and induce senescence.9, 12, 14, 15, 16 Several reports have described the up-regulation of TRF1, TRF2, and TIN2 in lung cancer,17 gastric cancer,...