For assessing RNA effects, we therefore employed 150 mM KCl low-salt buffer to homogenize the brain tissue, then treated the lysate either with or without RNase A before TRF2-S and FMRP Co-IP. Upon elimination of RNA, TRF2-S and FMRP were readily detected in their reciprocal immuno...
Cell 150, 481–494 (2012). 17. Loayza, D. & De Lange, T. POT1 as a terminal transducer of TRF1 telomere length control. Nature 423, 1013–1018 (2003). 18. van Steensel, B. & de Lange, T. Control of telomere length by the human telomeric protein TRF1. Nature 385, 740–743 ...
Accordingly, corticosteroids are commonly prescribed to alleviate asthmatic conditions [10], however, these elicit various undesirable side effects. Long term consumption of asthma controlled medications, such as anti-IgE or immunomodulators, by asthmatic patients was reported to cause development of ...
At 1650MHz,I tried a serial 3.0nH inductor at the modulator ouput as matching circuit, the OIP3 can be improved to about 24dBm at 0dBm output power(2-tone,space 150KHz,-3dBm each tone), but the bandwidth with 24dBm OIP3 is only about 40MHz. ...
TRF3761-K www.ti.com SLWS181K – OCTOBER 2005 – REVISED DECEMBER 2015 7.15.2 Typical Characteristics, TRF3761-B (See Figure 87) −60 −70 CL = 1651 MHz −90 −100 −110 −120 −130 −140 −150 −160 1k 10k 1M 100k −110 −120 −130 −140 10k 1M 100k ...
Labeled-DNA probes (0.5 ng) were incubated with 1 μg of purified baculovirus-expressed TRF1 protein for 20 min at room temperature in a 30-μl reaction containing 20 mm HEPES-KOH, pH 7.9, 150 mm KCl, 5% (v/v) glycerol, 4% (w/v) Ficoll, 1 mm EDTA, 0.1 mm MgCl2, 0.5 mm ...
From an EMI perspective, and since the synthesizer is typically a small portion of a bigger, complex circuit board, shielding is recommended to minimize EMI effects. 11.2 Layout Example Via to bottom ground BOTTOM GND De-coupling Capacitor's on back side of board BOTTOM GND TOP GND De-...
For standard conditions, TRF2 protein was diluted to a final concentration of 400 nM in the protein dilution buffer (50 mM HEPES, 150 mM KCl, pH 7.5) and incubated with an equal volume of T270 DNA (5.2 ng/μ l in same buffer: 50 mM HEPES, 150 mM KCl, pH 7.5) ...
Telomeres are nucleoprotein structures at eukaryotic chromosome termini. Their stability is preserved by a six-protein complex named shelterin. Among these, TRF1 binds telomere duplex and assists DNA replication with mechanisms only partly clarified. Her
Cells were lysed in IP buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.05% Triton X-100, 1 mM EDTA and EGTA, 10% glycerol, 1 mM DTT, and 1 mM phenylmethylsulfonyl fluoride), and supernatants were used for immunoprecipitation with Flag-M2-beads (Sigma). Beads were washed four ...