An engulfment assay: a protocol to assess interactions between CNS phagocytes and neurons. J Vis Exp. 2014;88:51482. 22. Chen M, Yang LL, Hu ZW, Qin C, Zhou LQ, Duan YL, et al. Deficiency of microglial Hv1 channel is associated with activation of autophagic pathway and ROS ...
The study protocol was approved by the Institutional Review Board (IRB) or Independent Ethics Committee (IEC) for each participating institution. All participants provided written informed consent, and the study was conducted in compliance with the ethical principles of Good Clinical Practice (GCP) as...
a Schematic representation of the experimental protocol. All experiments were performed on the indicated weeks. (B-E) Detection of cerebral blood flow changes by LSCI. b The field of view (also known as the ROI) was 5 × 5 mm. c Representative contrast and photographic images of cerebral ...
In vitro Ab stimulation and intracerebral Ab injection The oligomeric form of Ab1–42 was prepared according to a published protocol (Larson and Lesne, 2012). For in vitro Ab stimulation, primary microglia was seeded at a density of 2 Â 105 cells per ml into 24-well plates, and was ...
qRT-PCR Total RNA in cultured cells and brain tissues was ex- tracted using the RNAeasy™ animal RNA isolation kit with a spin column according to the manufacturer's protocol. Isolated RNA was reverse-transcribed into cDNA using the PrimeScript™ RT Master Mix (Perfect Real Time) following...