(Media=DMEM+pen/strep+15% horse serum+IL-2 (25U/ml)+IL-4 (20 ng/ml)). d. Antibodies were added at appropriate concentrations on day 0, 5, and 8. e. Cultures were incubated in 24 well Falcon tissue culture plates for 14 days. f. On day 14 supernatants were removed and assayed...
Add complete media with penicillin/strep, and place in the incubator. Remove the plate from the incubator and look at the well under the microscope. If you see excessive floating cells remove the media and wash once again with warm PBS. If few or no floating cells observed, incubate overnig...
(pen/strep, 100×), fetal bovine serum FBS mycoplex and ACCUTASE (a combination of protease and collagen in PBS with 0.5 mM EDTA) (from The Cell Culture Company PAA); trypan blue dye (from Merck); and CELLTITER-GLO luminescent cell viability assay (from Promega); bovine serum albumin ...
Sinusitis, enlarged nasal turbinates, tumors, infections, hearing disorders, allergic conditions, facial fractures and other disorders of the ear, nose and throat are diagnosed and/