Cell culture and treatment 3T3-L1 preadipocytes were maintained in DMEM sup- plemented with 10% NCS and 1% penicillin‒strepto- mycin (basal medium I, BMI). When the cells were confluent (Day 0), adipocyte differentiation was induced by treatment with a cocktail of 5 μg/mL ...
The streptococcal com system, historically the first QS-mediated behavioral program described in the literature [166], controls bacterial competence in numerous α-hemolytic streptocci. It consists of two com operons; the first encoding a protease and dedicated peptide export channel and the second ...