If you don’t use “trap” at all, terminating errors halt the command which is executing, and then the script moves on to the next command. In this example, you can see that the “terminating error” only terminates the command where it occurs, and execution continues with the next comm...
For adherent cells, at each time point, cells in a 6-well plate were washed with PBS and lysed in 400 μl RIPA lysis buffer (89900, Thermo) supplemented with Halt Protease Inhibitor Cocktail (78429, Thermo Fisher) and the Universal Nuclease (88702, Thermo Fisher). The lysate was ...
on the other hand, the BB or pellet may also be permitted to drop through the opening 32 in the lower plate 13b and into a collecting vessel positioned thereunder, but alternatively the provision of the opening may be dispensed with and the BB or pellet simply brought to a halt by the ...
Cells were harvested using—cell scraper and washed in phosphate-buffered saline (PBS), then lysed in 1 x RIPA Lysis and Extraction Buffer (Thermo Scientific #89901) containing Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific #1861281). Protein concentration was measured ...
2015) of dust traps, which is proposed as an efficient mechanism to halt or slow down the inward migration of dust grains toward the central star, offering a solution to the fast radial drift problem in planet formation theory (Whipple 1972; Weidenschilling 1977). To date, evidence of dust...
When a condition trap is currently disabled (OFF) and the specified condition occurs, the default action depends on the condition. For HALT and SYNTAX, the processing of the program ends, and a message (seeError numbers and messages) describing the nature of the event that occurred usually ind...
If you don’t use “trap” at all, terminating errors halt the command which is executing, and then the script moves on to the next command. In this example, you can see that the “terminating error” only terminates the command where it occurs, and execution continues with the next comm...
When a condition trap is currently disabled (OFF) and the specified condition occurs, the default action depends on the condition. For HALT and SYNTAX, the processing of the program ends, and a message (see Error numbers and messages) describing the nature of the event that occurred usually ...
Despite the recent progress, effective therapies to prevent, halt or even reverse DKD are lacking. Albuminuria, an earlier marker of DKD in most affected patients, indicates not only an increased risk for renal failure, but also cardiovascular complications [5]. A common underlying pathomechanism ...