英文名称:酸性磷酸酶, 白细胞(TRAP) Kit 387A-1KT 保质期:10年 个月 保存条件:低温 sigma-aldrich 是*的生命科学与高科技集团公司。我们的产品被广泛地应用于生命科学、有机化学、材料科学、分析化学与其他高科技的研发及生产。我们的用户遍及生命科学公司、大学与政府研究机构与企业,超过130万科学家与科研人员使...
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品牌:Sigma-Aldrich 所属分类:细胞生物学试剂 货品编号: Sigma.387A-1KT 品牌: Sigma 品名: 酸性磷酸酶, 白细胞(TRAP) 规格: 1KT Principle 将外周血或骨髓样品固定并制备显微镜玻片。制好的玻片随后孵育在萘酚AS- BI 磷酸及新鲜的 重氮化固深红GBC混合液中。 使用试剂盒来显示血液、骨髓及组织接触制片中的...
The differentiated HL-60 cells (dHL-60) were smeared on a clean slide glass using a cover glass, air-dried, fixed in 100% methanol for 5 min, and stained for 5 min with a Wright-Giemsa Staining Kit (K1438, Biovision, Milpitas, CA). Stained cells were rinsed with deionized water (...
A Cell Counting Kit-8 (CCK-8) kit was purchased from Dojindo Laboratories (Kumamoto, Japan), while a live/dead cell staining kit was obtained from Baxter Biotechnology (Shanghai, China). Triton X-100 and DAPI for cell staining were sourced from Sigma‒Aldrich. The radioimmunoprecipitation ...
Lipopolysaccharides (LPS) (Sigma-Aldrich) or PMA were directly added, and snapshots were taken at indicated times (e.g., 0, 15, 30, 45 min). Alternatively, according to the manufacturer and kit instructions, the reactions were measured for quanti- tative measurements of superoxides and ...
Cells were centrifuged at 1200 rpm for 5 minutes and supernatants were collected for ATP measurement using the ATP bioluminescent assay kit (#FLAA, Sigma Aldrich), according to the manufacturer’s instructions. For dye uptake assay, BMDNs were seeded in 384-well plate and maintained in a...
Next, PAECs were cocultured with neutrophils for 12 h in the inflammatory state of PAECs induced by silencing SelS, and SYTOX Green staining showed that the generation of NET was increased significantly (Fig. 4I and J) (P < 0.001). These results suggest that SelS may be involved in ...
ABC Elite for 30 min (Vector Laboratories), all at room temperature. ImmPACT DAB Kit (Vector Laboratories) was used to develop signals in accordance with the manufacturer’s instructions. Sections were counterstained with hematoxylin (Sigma). Rabbit anti-Cytokeratin 19 (Epitomics AC-0073; 1:...