In the compact and haploid genome of Dictyostelium discoideum control of transposon activity is of particular importance to maintain viability. The non-long terminal repeat retrotransposon TRE5-A amplifies continuously in D. discoideum cells even though it produces considerable amounts of minus-strand (...
Interval by which the instrument part should be transposed. Possible values range between a minor 2nd and a major 7th. A minus sign indicates a transposition downward; otherwise the transposition is upward. Alternatively, you can type a number between –11 and 11 to represent the number of half...
discoideum cells even though it produces considerable amounts of minus-strand (antisense) RNA in the presence of an active RNA interference machinery. Removal of the host-encoded C-module-binding factor (CbfA) from D. discoideum cells resulted in a more than 90聽% reduction of both plus- and...
Resting total isovolumic time was measured in seconds per minute: 60 minus total ejection time plus total filling time. The mean age was 31.6 plus or minus 7.6 years, and 38.0% were men. There were 16 patients (40%) who had more than or equal to moderate systolic dysfunction of the ...
intermediates when the minus-strand ssDNA donors were transfected in the presence of transposase (Fig.2c,lane 1) but not with plus-strand ssDNA donors (Fig.2c, lane 3). This result suggested that theHelraisertransposase is able to catalyze junction formation using a minus-strand ssDNA donor....
For control, we exploited this strand specificity by using the G to A ‘editing’ of the minus strand to test the significance of our results. Our data contains only the retrotransposon positive strands, thus, this event is represented as C to T ‘editing’ in our data. We present the ...
ELK1 transcription factors, coordinating pluripotency and self-renewal pathways, but suppressed by the KRAB-ZNF/TRIM28 epigenetic repression machinery, also known to regulate transposable elements. At the post-translational level, SUMOylation regulates HMGXB4, which modulates binding affinity to its prot...
In contrast, cytidine deamination of the minus strand DNA, as expected for APOBEC3 activity, would only enhance the frequency of G-to-A transitions on the plus strand. Because our analysis of endogenous L1 sequences showed a comparable number of substituted guanines and cytosines (Fig. 4), ...
The result states that for any orthogonal matrix Q (so QQ ⊺ = Q ⊺ Q = I) there exists a unique sign matrix D and a positive vector x such that Qx = Dx; a sign matrix is a diagonal matrix whose diagonal elements are equal to either plus one or minus one. The key ...
For this mutant, minus-strand synthesis is impaired and formation of the plus-strand strong-stop intermediate is eliminated. Intragenic second-site suppressor mutations of the transposition defect map to the RNase H domain of the enzyme. Our results demonstrate that one of the three active-site ...