HIV-1 Tat protein increases synaptic dopamine (DA) levels by directly inhibiting DA transporter (DAT) activity, ultimately leading to dopaminergic neuron damage. Through integrated computational modeling prediction and experimental validation, we identified that histidine547 on human DAT (hDAT) is ...
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Additionally, the distribution pattern of MRP2 in the GI tract is similar to that of the UGTs and GSTs, suggesting that this transport protein may act in concert with these conjugating enzymes to protect cells (Takano et al. 2006). The transport of substrates by MRP2 is complicated. A ...
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ABSTRACT: Glutathione S-transferases (GSTs) are a family of isozymes that catalyze the conjugation of glutathione (GSH), a tripeptide found in all mammalia... JP Veri,L Hermq,B Robaire - 《Journal of Andrology》 被引量: 226发表: 1993年 Effects of 3-beta-diol, an androgen metabolite wit...
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Express a glutathione S-transferase (GST) fusion NTR protein with a recognition sequence for thrombin or PreScission protease. • Bind the GST-NTR protein from the bacterial extract to GSH-Sepharose and cleave it with the appropriate protease. • Purify the recovered NTR protein on a Mono-Q...
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