We therefore employed primary mouse neonatal cardiomyocytes as a model system in which to test how cell-autonomous circadian regulation of ion abundance might impact upon cellular electrical activity. As with primary fibroblasts, we observed ~24 h cell-autonomous rhythms in intracellular Na+, K+,...
After cen- trifugation at 10,000 x g for 30 min (49, the TCA insoluble fraction was removed, resuspended in glass distilled water, and an aliquot counted in the Aquasol system. Parallel samples from the supernate and tissue free incubation media containing TCA were also counted. The ...
causing the particles to break into smaller fragments. This results in increasing particle numbers while the weight stays largely constant. Biofilm covering the surface of MPs can increase the density of otherwise floating MPs and cause them to sink. This seems...
In mammals, resveratrol is metabolized primarily as sulfated and glucuronidated products. A Caco-2 cell model was used in this study to observe the transport and uptake of resveratrol. GLUT1 was found to mediate resveratrol transport in the study. However, some studies have shown that GLUT1 ...
This in vitro system allows the screening of ABCB4-specific drug substrates and inhibitors independently of ABCB1 activity. Abcb1KO-MDCKII-ABCB4 cells constitute a reproducible, conclusive, and easy to use assay to study drug interactions with digoxin as a substrate. Screening a set of drugs ...
2D cell lines;ADME;differentiation;drug development;drug transport;intestinal epithelial cells;in vitro;jejunum;small intestine Graphical Abstract 1. Introduction The most common route for drug administration is the oral route [1]. Within the gastrointestinal system, the duodenum and jejunum play the mo...
Cerebellar slices from four male guinea pigs were used in this experiment. Incubation of 5.0 mm [1-13C]d-glucose and 0.5 mm [1,2-13C]acetate (control) with 10 mmol/L MeAIB (to block system A) [9,30]. Although MeAIB has been reported to block system L at 10 mm MeAIB there ...
(3) to explore the accumulation and transport patterns of PAEs derived from plastic film degradation agro-soil-plant system; (4) to provide a theoretical basis for assessing the acumulative risks of PAEs in soils and crops, evaluating the environmental effects of plastic film mulching and ...
determination of the particle number concentration, the TEF approach could be preferred as it might better account for particle losses in the sample introduction system. Keywords: single particle ICP-MS;transport efficiency;gold nanoparticles 2. Methods and Materials...
Versions Notes Abstract The sorption behavior of 2,4-dichlorophenoxyacetic acid (2,4-D) in the abundant agricultural volcanic ash-derived soils (VADS) is not well understood despite being widely used throughout the world, causing effects to the environment and human health. The environmental beh...