In current biology almost exclusively used to refer to a nucleotide sequence that directs the synthesis of a sequence complementary to it by the rules of Watson-Crick base-pairing. temporal sensing Mechanism of gradient sensing in which the value of some environmental property is compar...
but aORFs and CDSs for which we detected a peptide product are colored in dark blue or dark orange, respectively.BRibo-Seq landscape of the CDSs colored according to their protein abundance in ppm as provided by PaxDb [67].CVenn diagram of the number of aORFs detected with...
mRNA translation on the spindle is hypothesized to be an essential strategy for the localized production of cell regulators. This mechanism may be important particularly in early embryonic cells, which have a large diffusion volume and that undergo rapid
labeled Celf4+ neurons were observed in the preplate at E11.5 (the production peak for SP neurons) and in the SP at E13.5 (the onset of preplate splitting), some of which also co-expressed Nr4a2 and/or Ctip2 markers (Fig.3a). In contrast, prominent expression of Celf4 by SP neuron...
Hybridization and detection of the DIG labeled nucleic acid was performed using the DIG Easy Hyb and DIG Nucleic Acid Detection kits (Roche). Western blot Plant leaves were ground in Bradley buffer (50 mM Tris–HCl pH 7.5, 10 mM KCl, 20% glycerol, 0.4 M sucrose, 5 mM MgCl2,...
In order to obtain a quantitative value of perfusion using this approach with a single TI acquisition, the time duration of the labeled bolus must be known. This is achieved by applying saturation pulses to clearly define the bolus duration (using a QUIPSSII scheme, corresponding to the ...
RNA affinity pull-down using biotin-labeled RNA showed that only the GGGGCC repeats pulled down the DDX3X protein from cell extract, but the antisense CCCCGG repeats did not (Figure 4A), consistent with the specific effect of DDX3X on the sense repeat RNA translation (Figure 3A). We next ...
Hybridization and detection of the DIG labeled nucleic acid was performed using the DIG Easy Hyb and DIG Nucleic Acid Detection kits (Roche). Western blot Plant leaves were ground in Bradley buffer (50 mM Tris–HCl pH 7.5, 10 mM KCl, 20% glycerol, 0.4 M sucrose, 5 mM MgCl2,...
BMC Plant Biology 2012, 12:79 http://www.biomedcentral.com/1471-2229/12/79 RESEARCH ARTICLE Open Access The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis Kathryn Kamo1†, Ah-Young Kim2, Se Hee Park2...
Thanuja Krishnamoorthy et al., Molecular and Cellular Biology, vol. 21, No. 15, (Aug. 2001), pp. 5018-5030. Martin Bushell et al., The Journal of Biological Chemistry, vol. 276, No. 26, (Jun. 29, 2001), pp. 23922-23928. Tzann-Wei Wang et al., The Journal of Biological ...