The finished construct is amplified in bacteria, and the insert containing promoter, coding region, and polyA is isolated and purified. The purified fragment is then injected into the pronuclei of extracted fertilized oocytes harvested from a superovulated mouse mated one day prior to the extraction...
DNA. Once the recombinant DNA is incorporated into the bacterial host, the bacteria are allowed to replicate and clones are subsequently selected for recombination by meansof the antibiotic resistance incorporated into the plasmid vector. By growing the bacterial host on a medium containing antibiotic,...
To determine whether killing of bacteria was enhanced by expression of rat lysozyme, 5-wk-old transgenic mice and WT littermates were infected with 106 CFU of group B streptococci or 107 CFU of a mucoid strain of Pseudomonas aeruginosa by intratracheal injection. Killing of group B streptococci...
Bacteria are great for this because they grow and reproduce quickly and can be controlled quite well in the laboratory. GMOs can also be made by embryonic stem cell transfer, which involves growing stem cells in the lab, injecting them with a desired gene, and then reinserting them back ...
The rhizosphere is strongly influenced by plant-derived phytochemicals exuded by roots and plant species exert a major selective force for bacteria coloniz... Shirley,A.,Micallef,... - 《Plant Signaling & Behavior》 被引量: 141发表: 2014年 Microbial community composition explains soil respiration ...
Transgenic dairy cows that secrete lysostaphin into their milk have higher resistance to mastitis due to the protection provided by lysostaphin, which kills the bacteria Staphylococcus aureus, in a dose-dependent manner (Donovan et al. 2005). Lysostaphin is an antimicrobial peptide that protects the ...
An isolated polynucleotide comprising a sequence encoding the said protein, a plant cell that expresses the said protein, a genetically modified plant containing the claimed expression vector are also disclosed. Methods for preparation of the said plant, assessment of the stability of the said plant ...
A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. During the Human Genome Project, researchers had to find a way to reduce the entire human genome into chunks, ...
Interactions Between Bacteria and Algae in Aquatic Ecosystems Using a small substrate (thymidine 5'-(p-nitrophenyl phosphate) 3'-phosphate), the kinetics of staphylococcal nuclease insolubilized on CNBr-activated Seph... Cole,J J - 《Annual Review of Ecology & Systematics》 被引量: 1282发表: ...
The structure of an insect control protein gene from Bacillus thuringiensis var. kurstaki HD–1 was determined, and truncated forms of the gene that express a functional insecticidal protein were generated. Two of these truncated genes were incorporated