Transformation is the mechanism by which DNA is taken by bacterial cell from the environment. This phenomenon occurs in nature between bacteria under specific environmental conditions at low efficiency. Competency can be artificially introduced ...
Zolan ME, Pukkila PJ (1986) Inheritance of DNA methylation in Coprinus cinereus. Mol Cell Biol 6:195–200 Google Scholar Download references Author information Author notes Robert K. Dixon Present address: Environmental Research Laboratory, US Environmental Protection Agency, 97333, Corvallis, OR, ...
DNA will be released from the dead or damaged bacteria and can persist in the surroundingin vivoorex vivoenvironments for hours or even months [7]. Therefore, transformation can occur even if antibiotic resistance gene (ARG)-carrying antibiotic-resistant bacteria (ARB) are killed. The released AR...
The impact of homologous recombination on the generation of diversity in bacteria. J Theor Biol. 2006;239:210–9. Article CAS PubMed Google Scholar Lawrence JG. Gene transfer in bacteria: speciation without species? Theor Popul Biol. 2002;61:449–60. Article PubMed Google Scholar Pybus OG...
liquid as well as eye drops [143]. For example, after microwave sterilization, the bacteria, mold, yeast and escherichia coli in the decoction pieces of Euphorba officinalba were significantly reduced. After powder preservation, the bacteria and mold contained in the decoction pieces were all in...
density of the chromosome was displayed in the outermost layer of the circle. (C) The gradient colors and histogram show the number ofcis-elements in the promoter ofWOXgenes inE. grandis. The functional notes were enriched into four categories: light, plant growth development, phytohormone and ...
Transformation and surviving ofE. coliC600 have been evaluated in dependence on theelectric fieldstrengthandcurrent densityby changing the conductivity of the bacteria suspension. In this context the impact of making bacteria electrocompetent and the addition of NaCl solution was examined. ...
Genetic Engineering is a process that is used to the alter the genetic instructions in organisms. Restriction enzymes Are found in bacteria and are used to cut up DNA from a virus that might enter and take over the bacteria. They cut at specific sequences. ...
(MS) agar plate in the dark at 25 ± 1 °C for 48 h) with no pre-culture and then tested different vacuum infiltration times on transformation efficiency usingβ-glucuronidase(GUS) reporter system. Pre-culture increased the susceptibility of explants to theagrobacteriainfection and increased...
Transformation success was achieved by enzyme treatment that partially digested the cell walls of mature gametophytes to facilitate Agrobacteria infection. Co-incubation of Agrobacteria with enzymatically treated gametophytes was sufficient to generate transient transformants at a frequency of nearly 90% ...