To make selection vectors, a gentamicin resistance gene (gentamicin-3-acetyltransferase) driven by a salicylic-acid-inducible promoter was originally cloned into pGingerBS-NahR45and then variants were created for each of the pVS1, pSa and RK2 origins using a Gibson-like assembly with NEBuilder Hi...
The AE is represented by the proportion of the ligated fragment, which can be determined within 3?h. This qPCR-based measurement was tested by the commonly used restriction ligation, Golden Gate assembly, and Gibson assembly for the assembly of two or more DNA pieces; the results correlated ...
For transformation, 100 μl of cells were mixed with 100 ng of CSP, 0.04% BSA, 0.001 m CaCl2, and 100 ng of gene replacement cassettes made by Gibson assembly, or 300 ng of gene replacement cassettes made by restriction digestion and ligation. Transformation reactions were brought to a ...
(B) pKSEe401R was constructed in two steps: (I) cloning of the 158 bp OsMac3 5′-UTR fragment into pKSE401 using Gibson Assembly resulted in pKSEe401; (II) insertion of the DsRed1 cassette into pKSEe401 using T4 ligation resulted in pKSEe401R with the DsRed1 cassette in either ...
(Gibson 2012, pp. 68, 71). John McClure outlines an image of “preacher as host” where the preacher invites people to the table with their gifts, which are then placed by the host into dialogue with the word of God.27The host performs their duty by maintaining Christ and the mission ...
Gibson Assembly 克隆试剂盒可进行高效的无缝克隆,将不同长度的 DNA 片段克隆进入任意载体。 了解Gibson Assembly 克隆资源 对感受态细胞或细菌转化有疑问?点击以下资源,获取感受态细胞相关知识概览、视频、基因型和学习资源。 感受态细胞学习资源 查看细菌转化流程、故障排除、转化策...
Although the solution self-assembly of block copolymers has enabled the fabrication of a broad range of complex, functional nanostructures, their precise manipulation and patterning remain a key challenge. Here we demonstrate that spherical and linear su
Drawing on Butler, Bourdieu, Lefebvre, Gibson-Graham, and others, I make the case that the processes of recovering a business necessarily open the potential for new spaces of subjectivity, and that some workers, in the process of recovering their 32 work(places), have produced themselves as ...
senescence bypass in SHE clones. Further work is needed to confirm this regulatory phenomenon in Syrian hamster cells. In human cells, Bmi1 is known to regulatep16transcription both by direct binding to an upstream gene element and indirectly at a chromatin level by Polycomb protein assembly[29]...
Gibson Assembly 克隆试剂盒可进行高效的无缝克隆,将不同长度的 DNA 片段克隆进入任意载体。 了解Gibson Assembly 克隆资源 对感受态细胞或细菌转化有疑问?点击以下资源,获取感受态细胞相关知识概览、视频、基因型和学习资源。 感受态细胞学习资源 查看细菌转化流程、故障排除、转化策略和...