Having observed mitochondria transfer from the TME to mouse and human GBM models, we proceeded to interrogate the identity of the host mitochondria donor cells. GBM tumors in mouse models and humans are known to
The gut microbiome represents an important environmental factor that may exert an influence on symptoms, and a growing number of research groups have observed that children with ASD have distinctive gut microbiomes compared to neurotypical children7,8,9,10,11. Moreover, multiple mouse studies have ...
Several IRDS genes, including the transcription factor STAT1, influence RT/chemo resistance in cell lines and mouse tumor models. Interrogation across the most common human cancers revealed that a large proportion of untreated primary tumors express the IRDS. In breast cancer, IRDS expression measured...
ANOVA P < 0.001 Holm–Sidak multi-comparison test: **P < 0.01, ***P < 0.001, n = 3. i Renilla/firefly luciferase assay in neurons exposed to MVs pretreated or not with annexin-V (Annexin V), to limit MV–neuron contact. Kruskal–Wallis P < 0.001 Dunn’s multi-comparison test **P...
The cortices were mechanically dissociated, filtered with a 40 μm-cell strainer and cultured as neurospheres for 3–5 days in NeuroCult Proliferation media with epidermal growth factor (20 ng/ml). The neurospheres were collected and dissociated to single cells using a NeuroCult Chemical ...
Improved growth was observed for fish fed the FOS andP. acidilacticidiet when compared to the commercial control diet. However, it is not possible for us to evaluate whether the synbiotic treatment was the causative factor for the observed improvements in growth, since the experimental diets also...
Current clinically accepted technologies for cancer treatment still have limitations which lead to the exploration of new therapeutic methods. Since the past few decades, the hyperthermia treatment has attracted the attention of investigators owing to it
Two days after treatment cessation, EGFP+CD45− tumour stromal cells, excluding immune cells (CD45+), were isolated and sorted for scRNA-seq (Fig. 1a, b, Supplementary Fig. 1a, b). Eight stromal cell clusters were identified (Fig. 1c, d, Supplementary Table 1; Supplementary Fig. 1c...
They are vectors and the direction of the field can be considered as a leading factor in gene electrotransfer. It was indeed observed in vitro that the pattern of DNA interaction with cell surfaces was dependent on the field orientation25. We showed that 4 trains of HV-MV pulses delivered ...
×10/1/dilution. An antiserum was considered positive if its specific titer after immunization was at least 0.5 log10higher than the average of the preimmune titer. ELISPOT. Nitrocellulose-backed microtiter plates (96-well Multiscreen MA plate, Millipore St Quentin Fallavier, France) were coated...