(transfer buffer). when an electric field is applied, the proteins move out of the gel and onto the surface of the membrane, where the proteins become tightly attached. the resulting membrane is a copy of the protein patt...
The erythrocytes were lysed using RBC lysis buffer (R1010, Solarbio, Beijing, China). After 2 washes with staining buffer, cells were preincubated with Human TruStain FcX™ (422302, Biolegend, San Diego, CA, USA) for 10 min at room temperature, followed by staining with fluorochrome-...
The protein was transferred using a Bio-Rad Trans-blot Cell onto reinforced nitrocellulose membrane, in the presence of buffer containing 10% methanol. Sera were used at a dilution of 1:250 to specifically detect the mouse or rat IREG1 protein. After the primary antibody incubations (2 hr)...
The western blot membranes were stripped at each interval using mild stripping buffer (1.5% glycine, 0.1% SDS, 1% Tween-20 pH-2.2) for 15 min. at room temperature followed by re-blocking. Immuno-active bands were detected on a (Bio-Rad ChemiDoc-XRS, IL, USA) imaging system or under ...
PfSTART1 protein was dialysed extensively against 50 mM NaPO4-3 (pH 7.4), 150 mM NaCl, before diluting to 90 μM in the same buffer with 2% (v/v) DMSO (syringe sample). The first injection was 0.4 μL over a 0.8 s duration, and the remaining 19 injections were 2...
Noticeably, the CFC data obtained here allow for the definition of the establishment rates of the respective vectors in HSCs, presenting a platform for comparison amongst them, but they do not provide a proof for long-term establishment of the vector plasmids in HSCs, for which long-term cultur...
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Apoptosis, a form of programmed cell death, is critical for the development and homeostasis of the immune system. Chimeric antigen receptor T (CAR-T) cell therapy, approved for hematologic cancers, retains several limitations and challenges associated wi
brucei incubated with either PBS buffer alone (−), 3 μg RBP16, or 10 μg bovine histone in the presence of 2 μCi 3H-AdoMet in PBS overnight at room temperature. Left panel; Three micrograms of GST-PRMT7 was used as a positive control with each substrate for activity in vitro....
The transfer of intact mitochondria between heterogeneous cell types has been confirmed in various settings, including cancer. However, the functional implications of mitochondria transfer on tumor biology are poorly understood. Here we show that mitocho