In this chapter, we describe a method to enhance NK cell homing toward specific chemoattractants expressed in secondary lymphoid tissues through genetic modification of NK cells using mRNA electroporation. The method described here is scalable, cGMP-compliant, and offers a strategy to bolster the ...
Inflammatory Cytokine TNF-[Alpha] Inhibits [Na.Sup.+]-Glutamine Cotransport in Intestinal Epithelial Cells Kinetic studies revealed that TNF-[alpha] inhibited [Na.sup.+]-Gln cotransport by reducing the affinity of the cotransporters for Gln, and this effect was antagonized by genistein. Thus, we...
Natural killer (NK) cells can be engineered to kill resistant B-lymphoid cell lines and primary B-cell chronic lymphocytic leukemia (B-CLL) cells after transfection with chimeric antigen receptors (CARs) recognizing CD19 or CD20. Here we compared mRNA electroporation with lentiviral vector (LV) ...
NK cells expansion was directly proportional to the number of UC-MSC plated as feeders. Expanded CB-NK cells also displayed enhanced cytotoxicity compared to control cultures plated with cytokines only (91.78±0.7% vs. 82.5±1.8%, p=0.003 and 89±2.3% vs. 83.7±0.18%, p=0.056, respectively...
(c) Effect of mRNA concentration on mRNA VECT transfection of NK cells (Mean and individual values, n = 3). Significant differences were found in relative viability (Welch ANOVA test, P = 0.435), but not transfection efficiency (Welch ANOVA test, P = 0.2793), across the ...
The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their efficacy has been variable and is highly dependent on the ce...
“Application of jetMESSENGER reagent to introduce modified RNA molecules into primary lung mouse endothelial cells (a cell population which is generally hard-to-transfect with DNA/RNA using non-viral methodology) resulted with the highest transfection rate (>70%) and with the lowest toxicity effects...
Role of NK and T cells in IL-12-induced anti-tumor response against hepatic colon carcinoma. IL-12 is an immuno-regulatory cytokine that has been shown to generate a potent NK and Th1 response in a variety of laboratory models. However, the detailed immune development in the hepatic tumor ...
of CD117 (stem cell factor, SCF, receptor; c-kit) and CD25 (IL-2Rα) (3). The earliest population is identified as CD117+CD25−, and contains precursors for T, B, and natural killer (NK) lymphocyte lineages. Induction of CD25 expression on progenitor CD117+thymocytes characterizes ...
Region 1 peptides, B1-1 (0014Lys-Glu0054) and B1-2 (0055Leu-Ala0096), mediate transfection of HeLa cells but are cytotoxic. Region 2 (3313Asp-Thr3431), containing the known B/E receptor ligand, shares analog motifs with the human herpesvirus 5 immediate-early transcriptional regulator (UL...