Lemoine, N.R., and Wynford-Thomas, D. (1990). Transfection and transformation of human thyroid epithelial cells. In Animal Cell Cul- ture, J.W. Pollard and J.M. Walker, ed. (Clifton, NJ: Humana Press), pp. 565-572.Lemoine NR, Wynford-Thomas D 1997 Transfection and trans- ...
(Lentiviral vector containing sh-NUDC-F can be successfully packaged in 293Tcells and then efficiently transduced into Damicells to silence hNUDC gene. )这三个例句是笔者在从事编辑工作时遇见的,作者原先的翻译都是 transfected,笔者将例 2 改为 transformed,例 3 改为 transducted。单从中文原文很难...
The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their efficacy has been variable and is highly dependent on the ce...
cells include viral delivery (commonly known as transduction), and transformation – using plasmids to deliver DNA into cells. In all cases, the process involves increasing the permeability of the recipient cell’s membrane to allow for exogenous genetic material to pass into the cytoplasm. ...
The transfer of foreign genetic material from one organism to another is one of the pillars of modern-day genetic engineering, with applications from knocking out genes to protein production. However, there are several ways to transfer foreign nucleic acid into cells. Transformation, transfection, a...
Transfection is a process by which foreign DNA is transferred to host cells. It includes any method for gene delivery but is usually used in the context of non-viral methods. Transfection can result in transient or stable genetic changes to the host (transformation). ...
Lavitrano, Mariauisa et al., Sperm Cells as Vectors for Introducing Foreign DNA Into Eggs: Genetic Transformation of Mice, Cell, vol. 57, pp. 717-723, (Jun. 2, 1989). Mittereder et al., Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy, J. Virology...
The nicking of the plasmid results in markedly reduced efficiencies in transformation ofand transfection of HEK293T cells. Thus, this work reveals yet a new mechanism for the antimicrobial activity by protease inhibitors... MR Islam,K Ihenacho,JW Park,... - 《Scientific Reports》 被引量: 0发...
Background聽聽The use of lentiviruses to reprogram human somatic cells into induced pluripotent stem (iPS) cells could limit their therapeutic usefulness d... Karim,Si-Tayeb,Fallon,... - 《Bmc Developmental Biology》 被引量: 256发表: 2010年 Transformation and Oncogenesis by Jaagsiekte Sheep Retrov...
Exposure to chemical carcinogens or radiation is considered to cause most human cancer, but human cells in culture have not been successfully transformed to malignancy by such agents. Malignant transformation is a multi-step process and one explanation for the failure to induce such transformation of...