Read-through transcription ofSVKresults inasCBF1 To better understand the mechanism ofSVKrepression, we revisited the effects of theuns-1insertion.uns-1is inserted downstream ofSVKyet results in equivalent cold-related defects compared tosvk-1. A possible explanation for the effects seen in thesvk-...
Here, we have directly tested the transcriptional read-through mechanism. Contrary to previous suggestions, we show that transcription through the PRE is not sufficient for inducing an epigenetic switch in PRE activity. In fact, even high levels of continuous transcription through a PRE fails to ...
An alternative explanation for proviral silencing is that transcriptional interference, characterized by occlusion or dislodgement of transcription initiation or elongation complexes from the provirus by readthrough transcription of the viral-containing host gene or termination of host-initiated transcripts at...
polyadenylation, and detachment of the RNA pol II machinery31, is induced in the host by HSV-1 infection of human foreskin fibroblasts19and has been posited as another mechanism by which the infecting virus disrupts host gene expression. While that study found no evidence of read-through transc...
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Discovery and overproduction of novel highly bioactive pamamycins through transcriptional engineering of the biosynthetic gene cluster Nikolas Eckert, Yuriy Rebets, Lilya Horbal, Josef Zapp, Jennifer Herrmann, Tobias Busche, Rolf Müller, Jörn Kalinowski & Andriy Luzhetskyy Microbial Cell...
Human immunodeficiency virus type 1 (HIV-1) also has been reported to induce DNMT1 through the responsive element residing in the -1634 to +71 of DNMT1 promoter [45]. The increase in expression of DNMT1 and overall genomic methylation as well as hypermethylation of the p16INK4A gene are ...
Controversial studies reported the role of SFN in potentiating colon tumorigenesis through the activation of various factors, such as matrix metalloproteinase 28 (MMP28), which contributed to the progression of colorectal cancer. Therefore, tezacaftor probably repressed the expression of the SFN gene ...
(RNA-seq) data from naive mouse T cells, where 3′-UTR shortening is widespread and associated with the transition of the cells from the naive to activated state7. Manual examination of RNA-seq data12revealed many genes with a distinctive gap in their read coverage along the 3′-UTR, ...
5 min, 8 min and 10 min were used for reactions done at 100 μM NTP. A chase reaction was done by adding 1 mM NTP. The half-life of transcriptional pausing was done by determining the fraction of each RNA species compared with the transcription readthrough for each time ...