Transcription regulation constitutes a pivotal phase in gene expression, significantly influenced by various factors such as high-order chromatin structure, chromatin remodeling, DNA methylation, histone modification, and non-coding RNAs. In an effort to foster academic communications and collaborations within...
Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix these issues. Here, we use...
d, Tagging endogenous TFs with mNeonGreen/GFP by CRISPR/Cas9-mediated gene knock-in. A homologous recombination (HR) template sequence containing the coding sequence of mNeonGreen/GFP (green) flanked by 800-1,000 bp sequence homologous to the endogenous TF locus was used to guide HR ...
[Foundation.Register("SFTranscriptionSegment", true)] [ObjCRuntime.Introduced(ObjCRuntime.PlatformName.iOS,10,0, ObjCRuntime.PlatformArchitecture.All, null)]publicclassSFTranscriptionSegment:Foundation.NSObject,Foundation.INSCopying,Foundation.INSSecureCoding,IDisposable ...
Transcription of a protein-coding gene on B chromosomes of the Siberian roe deer (Capreolus pygargus) Vladimir A Trifonov, Polina V Dementyeva, Denis M Larkin, Patricia CM O’Brien, Polina L Perelman, Fengtang Yang, Malcolm A Ferguson-Smith & Alexander S Graphodatsky BMC Biology...
23,24 Although CPF processes the 3′ ends of protein-coding (pc) transcripts, the related associated with Pta1 (APT) complex is required for the transcription of non-coding small nuclear (sn)RNAs and small nucleolar (sno)RNAs by RNA Pol II in yeast.25,26 APT contains all six subunits ...
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These changes of domains were analyzed based on the coding regions of alternatively spliced exons (See Additional file 3). Conclusion In this study, HUVECs were incubated with 300 μM CoCl2 for 24 hrs to induce the balance between cell survival and apoptosis, followed by a genome-wide ...
which is very useful because it enables simultaneous detection of several pathogens by introduction of several different primers to amplify DNA regions coding for specific genes of each targeted bacterial strain; reverse transcriptase PCR (RT-PCR); and real-time RT-PCR (Desai et al., 2008; Malor...
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