and lacks genes derived from the NX element. The divergence between clade I and the Rhabditida is dated at 400 million years ago54. During this time, either separate sex chromosomes originated independently in clade I and the ancestor
A real-time reverse transcription-PCR method was developed to determine whether the recovery of culturability of viable but nonculturable (VBNC) Vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type III secretion system 2 (TTSS2). ...
Transcription of a protein-coding gene on B chromosomes of the Siberian roe deer (Capreolus pygargus) Vladimir A Trifonov, Polina V Dementyeva, Denis M Larkin, Patricia CM O’Brien, Polina L Perelman, Fengtang Yang, Malcolm A Ferguson-Smith & Alexander S Graphodatsky BMC Biology...
To help distinguish DPC-specific effects from those caused by other formaldehyde-induced lesions, we screened in parallel with 5-aza-dC, which specifically cross-links DNMT1 to DNA (Fig. 1a). Using a false discovery rate (FDR) cut-off of 0.1, we identified 93 protein-coding genes whose ...
The time course of synthesis of snowshoe hare bunyavirus small (S)- and medium (M)-sized viral RNA (vRNA), viral cRNA (vcRNA), and mRNA species was analyzed by using single-stranded DNA probes representing the S- and M-coded gene products. In the presence of puromycin, an inhibitor ...
[Foundation.Register("SFTranscription", true)] [ObjCRuntime.Introduced(ObjCRuntime.PlatformName.iOS, 10, 0, ObjCRuntime.PlatformArchitecture.All, null)] public class SFTranscription : Foundation.NSObject, Foundation.INSCopying, Foundation.INSSecureCoding, IDisposableEredit...
which is very useful because it enables simultaneous detection of several pathogens by introduction of several different primers to amplify DNA regions coding for specific genes of each targeted bacterial strain; reverse transcriptase PCR (RT-PCR); and real-time RT-PCR (Desai et al., 2008; Malor...
We identified a pair of miRNAs that target protein-coding genes involved in the defense response against fungal pathogens; Md-miR156ab targets a novel WRKY transcription factor, MdWRKYN1, which harbors a TIR and a WRKY domain. Md-miR395 targets another transcription factor, MdWRKY26, which ...
At any point \({{{\boldsymbol{x}}}\) in the nucleus, at a time \(t\), we consider three nuclear constituents, namely the nucleoplasm and the two phases of chromatin, euchromatin and heterochromatin with their volume fractions (refer Supplementary Information SI, Supplementary Section S1.1 fo...
Prey plasmids were generated by fusing the coding sequences of αFTZ-F1T288A(-P) and αFTZ-F1T288D(P) into the pGADT7 vector, which were then introduced into the bait strains and selected on SD/-Leu medium with AbA. The yeast co-transformed with the pGADT7-p53 and pAbAi-p53 ...