synthesis inhibitor hydroxyurea (HU) can induce replication stress to slow replication elongation and thus greatly improve the resolution of EdU-marked DNA replication initiation (EdU-seq-HU) experiments [13,15]. Small nascent DNA strands (SNS) have been used to map DNA replication origins [21,22...
If PARP1 or TIMELESS and TIPIN fail to perform their function, TRCs lead to DNA damage that requires HR for repair. The main difference between the two proposed mechanisms is the nature of the object with which the replisomes collide: trapped PARPs versus transcription elongation complexes. ...
h, Per gene RNAPII occupancy versus DNA accessibility, as determined via ATAC-seq in RPE1 cells (GEO: GSE209659). i, The same as g but only showing genes that show transcription-dependent DPC repair (as defined in g) and a group of matched size that do not show transcription-dependent ...
Both thumb and fingers are hyperextended, owing to the wider dsRNA versus RNA/DNA and dsDNA. Unexpectedly, the RTIC active site region is most similar to that in RT/dsDNA/NNRTI, albeit differing widely in their nucleic acid tracks and contacts with RT [7••]. The RTIC crystal ...
The fidelity of reverse transcription differs in reactions primed with RNA versus DNA primers.reverse transcriptasenucleotide misincorporationreverse transcription fidelityRNA-DNA and DNA-DNA duplexesretroviral replicationReverse transcriptase enzymes (RT) convert single-stranded retroviral RNA genomes into ...
Nuclear Factor I (NFI) transcription/replication proteins are essential for both Adenoviral DNA replication [1–3] and for the regulation of transcription throughout development [4]. There are 4 NFI genes in mammals (Nfia, Nfib, Nfic and Nfix) and single NFI genes in Drosophila, C. elegans,...
Various factors complicate the comparison of nucleosome levels at different stages: (1) the ratio of human versus P. falciparum DNA changes during the cell cycle as the parasite multiplies its genome while the amount of human cells is invariant. Accordingly, the fraction of human sequence reads ...
the level of DamMT can influence cellular functions such as gene transcription, DNA mismatch repair, initiation of chromosome replication and nucleoid ... Lbner-Olesen A,O Skovgaard,MG Marinus - 《Current Opinion in Microbiology》 被引量: 438发表: 2005年 Chromatin loop domain organization within ...
The late transcripts appear after the onset of viral replication and are extended to their full lengths towards the end of the approximately 8.5 h cycle. While we detected only small differences in genome-wide analysis of the host Sulfolobus solfataricus comparing infected versus uninfected strains,...
(Fig.5b). These observations suggest that a non-nucleolytic function of MRE11 is important for R-loop regulation. To gain more insights into this, we monitored the activity of the MRN nuclease on DNA/DNA versus DNA/RNA hybrids (Fig.5c). The MRN nuclease activity was very efficient on ...