Investigations toward the selection of fully-modified 4'- thioRNA aptamers: Optimization of in vitro transcription steps in the presence of 4'-thioNTPs. Bioorganic and Medicinal Chemistry. 2008; 16:9450-9456. [
We amplified all 40-amino-acid tiles in steps of 20 amino acids starting from the first amino acid of the sequence using the primers listed in Supplementary Table 6. The amplified gene fragments were cloned into a pGAL4 backbone, linearized with AsiSI (NEB) and BsiWI (NEB), via NEBuilder...
To understand genome function, the regulatory steps of gene transcription must be defined and it must be analyzed under which conditions they become rate limiting. A rate-limiting step may be defined as the slowest molecular transition in the process that limits the overall progression and the tra...
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RNA in disease Know about CRISPR Cas9 technology in gene editing and its application in human therapeutics to agricultureExamining how scientists attach the molecular tool CRISPR-Cas9 to an RNA strand in order to edit genes and repair damaged DNA sequences.(more) ...
The other 5′-cap selection technique, the oligo-capping method, consists of three steps of enzymatic reactions (Bai et al., 2014; Maruyama & Sugano, 1994). First, the phosphate residue in the 5′-ends of truncated (eg, partially degraded) mRNAs that are noncapped is hydrolyzed by bacteri...
This is a common scenario in motif discovery, where the set of known sequences belonging to a given binding motif is incomplete. In order to simulate this situation, we proceeded in a similar way to our previous experiment; all the steps were the same except that we gave a single base ...
multiple processes including exopolysaccharide biosynthesis and motility [30–32]. In order to map ExpR-dependent transcripts, which would otherwise be lacking, RNA from anexpR+derivative ofS. meliloti1021 was also included in our pool. Enzymatic treatment of RNA samples with terminator 5′-phosphate...
This refactoring provides a more efficient and swift path for RDMA operations in data center networks. Firstly, we handle the CQ, then the EQ, and finally the interrupt. We didn’t make any modifications to these three steps because they originate from the NIC hardware, and we can’t ...
The introduction of these amplification steps during any RNA detection assay increases the risk that some RNA species are preferentially amplified over others. To prepare libraries representing an mRNA population, the use of poly-thymidine primers to amplify cDNA from purified RNA leads to a ...