Covalent DNA–protein cross-links (DPCs) are toxic DNA lesions that block replication and require repair by multiple pathways. Whether transcription blockage contributes to the toxicity of DPCs and how cells respond when RNA polymerases stall at DPCs is unknown. Here we find that DPC formation arre...
The mNET-seq library was obtained by PCR for 12–14 cycles. For mNET-seq analysis, we wrote a customized pipeline to process raw data as follows: (1) adapter trimming: we used TrimGalore (v.0.6.7) (https://github.com/FelixKrueger/TrimGalore) to remove sequencing adapters ‘AGATCGGAAG...
as Rev in the punctuation department. It also kept all the filler words like "um"s and "uh"s in the transcript, which other apps seemed to remove automatically. (Descript's AI underlord does have a "Remove filler words" feature, but it feels like just an unnecessary additional step.) ...
In this post, we walk you through the process of training and deploying a churn prediction model onAmazon SageMakerthat usesHugging Face Transformersto find useful signals in customer-agent call transcriptions. In addition to textual inputs, we show you how to ...
SuperScript Reverse Transcriptase Overview: cDNA Synthesis with Superscript IV RT Overview: SuperScript One-Cycle cDNA Kit Overview: Basic Principles of RT-qPCR Overview: Reverse Transcription Guide: PCR & Molecular Cloning Handbook Comparison: One-Step & Two-Step...
The transcription process just doesn't continue. Nothing happens after clicking "Transcribe sequence", or transcription progress just stops for no reason. Language is just English. Been using it before in a normal way. Yes I did installed Premier Pro ...
If you are running these in a notebook environment, and this is your first time installing these packages, you will need to shut down this notebook kernel by going toKernel — Shut Down Kernel,and then reopen the kernel to make sure all the packages are recognized in the notebo...
A process through which the RNA polymerase slows down transcription during elongation. 5′-Rapid amplification of cDNA ends (5′-RACE). A method to amplify mRNA between a defined internal site and its initiation site. Global regulators Transcription factors that affect a large number of genes invo...
We discovered that this process is required for nuclear translocation and transactivation. The phase transition is driven by interactions between aromatic residues, distributed throughout the sequence of the AD but particularly concentrated near the C terminus. This region includes a subdomain of the ...
Autophagy is a multistep process that determines the engulfment of cytoplasmic components, such as protein aggregates, damaged organelles, and cell debris in double membrane vesicles called autophagosomes (Fig.1). These vesicles fuse with the lysosomes, membrane bound organelles characterized by an aci...