This article describes a procedure to prepare a raft-like intracellular membrane fraction enriched for the trans-Golgi network (TGN) and endosomal compartments. The initial step in this technique involves cell
The Golgi complex receives nascent proteins that have been carried from the ER in vesicles. Multiple membrane stacks, transport vesicles, and the trans-Golgi network (TGN) form the Golgi complex. The cis-Golgi is closest to the nucleus, whereas the trans-Golgi is the most distal. Unlike the...
Multiple membrane stacks, transport vesicles, and the trans-Golgi network (TGN) form the Golgi complex. The cis-Golgi is closest to the nucleus, and the trans-Golgi is the most distal. Unlike the ER, the cisternae of the Golgi stacks are not contiguous and their widths are not uniform. ...
TGN exportThe sorting and distribution to different final destinations of roughly a third of the membrane and secreted proteins occurs at the level of the Trans〨olgi Network (TGN). This TGN mission involves efficient mechanisms of cargo recognition and activation of specific signaling pathways. This...
SNX6-associated vesicles contact PtdIns(4)P-positive Golgi membrane in another region of the cell. 3D-SIM movie (DeltaVision OMX V3) of another enlarged region of interest from the cell (Fig. 1e) shows contact between PtdIns(4)P-enriched Golgi membrane (green) and SNX6-associated vesicles ...
Furin was found predominantly in the trans-Golgi network (TGN), but also in clathrin-coated vesicles dispatched from the TGN, on the plasma membrane as an integral membrane protein and in the medium as an anchorless enzyme. When furin was vectorially expressed in normal rat kidney (NRK) ...
Consortin is located at the trans-Golgi network (TGN), in tubulovesicular transport organelles, and at the plasma membrane. It directly interacts with the TGN clathrin adaptors GGA1 and GGA2, and disruption of this interaction by expression of a consortin mutant lacking the acidic cluster鈥揹...
Glombik MM, Kromer A, Salm T, Huttner WB, Gerdes HH. The disulfide-bonded loop of chromogranin B mediates membrane binding and directs sorting from the trans-Golgi network to secretory granules. Embo J 1999;18:1059-1070.Glombik, M. M., Kromer, A., Salm, T., Huttner, W. B. and ...
p230/golgin-245 is a trans-Golgi coiled-coil protein that is known to participate in regulatory transport from the trans-Golgi network (TGN) to the cell surface. We investigated the role of p230 and its interacting protein, microtubule actin crosslinking protein 1 (MACF1), in amino acid ...
Formation of transport vesicles requires the coordinate activity of the coating machinery that selects cargo into the nascent vesicle and the membrane bending machinery that imparts curvature to the forming bud. Vesicle coating at the trans-Golgi Network (TGN) involves AP1, GGA2 and clathrin, which...