extracellular vesicles formed by the ESCRT machinery and constitutively released into the media (referred to herein as EVs) can be collected from the culture supernatant of activated T cells16. Previous work has used EVs as a surrogate for tSV. While this approach has had some predictive power,...
Urinary miR-16 was induced earlier than traditional kidney injury marker, urea or creatinine, in a mouse I/R renal failure model. To delineate whether urinary miR-16 is related to the kidney, the content of miR-16 in various mouse organs was measured. Using semi-quantitative qPCR, we ...
Marker, Dim2, Dimethyl Histone H3, DIO3, DIP2B, DISP1, Distal-less, Dkk-1, DLEU7, DLK, DLK2, Dlx-1, Dlx-2, Dlx-4, DMBT1, DMP-1, Dmp1, DMRT1, DMRTA2, Dmrtc1c, DMXL2, DNA pol ε cat, DNAAF3, DNAH1, DNAH10, DNAH11, DNAH12, DNAH17, DNAHC12, DNAHC7A, DNAHC7B...
The selectable and amplifiable marker genes do not have to lie immediately adjacent to each other. The amplifiable marker gene and selectable marker gene can be the same gene. One or both of the marker genes can be situated in the intron of the DNA construct. Suitable amplifiable marker gen...
M – DNA-Molekulargewichtsmarker VI (Boehringer Mannheim). Für 12a: Spur 1 – neutrophile Zellen (reif), Spur 2 – Muskel, Spur 3 – Thymus, Spur 4 – Herz, Spur 5 – Nebenniere. Für 12b: Spur 1 – Niere, Spur 2 – Placenta (8 Wochen), Spur 3 – Placenta (11 Wochen), ...
et al. ICAM1 is a potential cancer stem cell marker of esophageal squamous cell carcinoma. PLoS ONE 10, e0142834–e0142834 (2015). Article PubMed PubMed Central CAS Google Scholar Schaefer, C. F. et al. PID: the pathway interaction database. Nucleic Acids Res. 37, D674–D679 (...
The invention provides a therapeutic mRNA composition and methods for administration, resulting in highly efficient and sustained protein production in vivo and successful reduction of, for example, ammonia accumulation in blood, orotic acid levels in urine, and/or any clinically relevant disease marker...
2. An integrative plasmid for cloning DNA in Mycobacterium spp. comprising: a) the first DNA sequence of claim 1a), wherein said heterologous DNA of interest is under the control of a mycobacterial promoter region; b) an E. coli origin of replication; and c) a selective marker for mycobac...
To make such conjugates effective, a monoclonal antibody delivering such toxic agents must be able to bind to its target antigen and internalize into cells to carry the toxic agent inside where it can be effective at damaging DNA or inhibiting protein synthesis or other metabolic functions of the...
For CAPS marker genotyping, gDNAs of 20 cultivars were extracted as described inSection 4.4. WGRS based on NGS. PCR was performed in a total volume of 20 µL containing 10 ng of genomic DNA, 0.3 µM each forward and reverse primer, 1× PCR buffer, 0.2 mM dNTPs, and 0.5 U of ...