3b) at these positions did not detectably affect activity, and results in an inter-dye distance of 34–57 Å if the RNAP and the ribosome are in a coupled state (Extended Data Fig. 3a). Fig. 3: Real-time tracking of ribosome–RNAP coupling in dependence of intervening mRNA length...
LC–MS and SDS–PAGE was performed to assess purity and integrity of all purified or modified proteins. A customized Bradford assay (no. 5000006, Bio-Rad Protein Assay; Bio-Rad Laboratories Inc.) was used to determine protein concentrations and single-use aliquots were flash frozen in liquid ...
1a). Furthermore, biochemical characterisation of RING1B-HaloTag protein revealed that it was incorporated into PRC1 complexes in a manner that was indistinguishable from the wild type protein (Fig. 1c and Supplementary Fig. 1b, c). When a HaloTag compatible dye (JF549)66 was applied to ...
Molecular-weight markers (Bio-Rad, Hercules, CA, US) are indicated in kilodaltons. (B) SDS-PAGE gel of the three purified GSTe2 alleles (26.8 kDa). (C) Topology of GSTe2 showing the C- and N-terminals, the GSH binding pocket (G-site) and the substrate-binding pocket (H-site). ...
The protein purity was assessed by visualization on SDS-PAGE gel. 2.7. OX40 antigen-specific memory T cell assay Antigen-specific CD4 T-cells responding to recall antigens were measured in cultures of 300,000 PBMC in 200 μl/well of a 96-well plate, in Iscove's Modified Dulbecco's ...
2A). The presence of HIV-1 gag VLPs in this fraction was confirmed by SDS-PAGE (Fig. 2B), Western blot (Fig. 2C), and TEM (Fig. 1B, C). SDS-PAGE (Fig. 2B) clearly demonstrates the presence of bands at 55 kDa, which were identified as gag polyprotein by Western blot analysis...
dye across multiple organs, including for labeling cells for times as long as days)12,13,14,17,18. The durability of SPACECAT’s fluorescent signals will depend on sample characteristics (e.g., longer signal duration in slowly dividing cells, shorter duration in metabolically active cells). ...
Straight-channel chip is filled with dye for a better visibility. Both the serpentine (Figure 2a) and straight-channel (Figure 2b) chips have the same H-cell design. The polymer solution and solvent are infused into the lower and upper input channels, respectively (Figure 2c). At the ...
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