Increase in cell number from embryo (12 cells per hemisegment) to third instar (up to 50 cells per hemisegment) is the result of proliferation of a single ePG that serves as transient progenitor and only contributes to the outermost perineurial glial layer.doi:10.4161/fly.28855...
Here, we apply single-cell sequencing to identify and trace the origin of DTCs in breast cancer. We sequence the genomes of 63 single cells isolated from six non-metastatic breast cancer patients. By comparing the cells' DNA copy number aberration (CNA) landscapes with those of the primary ...
6. Processing and analysis of single-cell RNA-seq data ,生信工程师上场,原文写得非常非常详细。 框架: (1) Single-cell RNA sequencing of salivary gland tumors. (2) A comprehensive salivary gland cell atlas. (3) Identification of cancer stem cells and other tumor-specific cell populations...
因为通过state manifolds鉴定到的Dynamic trajectories 其实是hypothetical,具体而言:处于branch-points的细胞的 cell division may or may not occur, and sister cells from each division may both progress along one branch of a manifold, rather than exploring all branches (Fig2a). 但是在真实的cell lineage t...
although they expressed secretory cell genes such asScgb3a2(Fig.1f, gand Supplementary Data2). Importantly, DTA+cells did not have a higher percentage of mitochondrial genes or a lower number of counts or genes per cell, supporting the notion that these are viable cells (Supplementary Fig.1h...
Neuronal death as a regulative mechanism in the control of cell number in the nervous system The growth of the processes of dissociated hippocampal neurons from 18- to 20-day-old rat fetuses has been studied in vitro using a method that permits cells to be maintained at relatively low plating...
Recent lineage tracing studies using Apln-CreER tool indicated that a large number of post-natal coronary vessels do not form from pre-existing vessels. Instead, they form de novo after birth, which represents a coronary vascular population (CVP) distinct from the pre-existing one. Herein, we ...
A new triangular-grid ray tracing (TGRT) method is proposed. It is based on dividing the propagation region into triangles. The number of triangles is decided by the number of vertices of the structures, instead of their dimensions. For the 2D case, it can decreases the visible decision calc...
摘要: The search for an understanding of the biological bases of learning and memory requires the consensus of results from a number of techniques including recording, stimulation, pharmacology, anatomy and, not the least, the lesion. No one technique, in isolation of the...
Using helper viruses with cell type specific promoters to target retrograde infection of pseudotyped and genetically modified rabies virus evidence was found for direct synaptic input onto V1 inhibitory neurons. These inputs were similar in number to geniculocortical inputs and, therefore, considered ...